Determination of Appropriate Compost Based Nursery Media Preparation for Pineapple (Annanas comosus MERR L.) Seedling Growth at South Ethiopia
Issue:
Volume 6, Issue 2, December 2021
Pages:
14-20
Received:
5 July 2021
Accepted:
19 July 2021
Published:
24 July 2021
Abstract: The experiment was conducted at Awada Agricultural Research Center found at south Ethiopia to determine a proper ratio of compost to top soil ratio that enhanced growth of pineapple at seedling stage. The experiment was arranged in randomized complete block design (RCBD) with three replication. Seedlings were raised from ground slip and planted in plastic pots with width to height (16x22cm) containing compost to topsoil in 0:1, 1:1, 1:2, 1:3, 1:4 and 1:0 ratios as treatments. Seedlings were watered as required and grown for 6 months. determination of pineapple seedling height, leaf length, leaf width, leaf area, leaf fresh weight, leaf dry weight, root number, root dry weight and total plant dry biomass. Data were analyzed using SAS software and means were separated using LSD at 5% probability. The results revealed significant variation among the treatments. All the recorded growth parameters showed progressive increase with increase in the proportion of compost. The compost based nursery media preparation from compost (1:0) and or compost to top soil in one to one (1:1) ratio mixture is performed the best growth of pineapple seedling in terms of plant height, leaf length, leaf width, leaf area, leaf fresh weight, leaf dry weight, root number, root dry weight and total plant dry biomass. Therefore, it is recommendable that compost and or compost to top soil in one to one (1:1) ratio mixture media could be used to grow the best performed pineapple seedling in south ecological zone at nursery level via promoting production and utilization of compost from cheaper locally available materials before transplanting to its production field.
Abstract: The experiment was conducted at Awada Agricultural Research Center found at south Ethiopia to determine a proper ratio of compost to top soil ratio that enhanced growth of pineapple at seedling stage. The experiment was arranged in randomized complete block design (RCBD) with three replication. Seedlings were raised from ground slip and planted in ...
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Properties of Partially Purified Rhodanese from Leaves of Cassava in Owo Southwestern Nigeria
Omowumi Funke Adedugbe,
Oluwatosin Desola Owolala,
Olutosin Samuel Ilesanmi
Issue:
Volume 6, Issue 2, December 2021
Pages:
21-25
Received:
10 August 2021
Accepted:
25 August 2021
Published:
19 November 2021
Abstract: Introduction: Rhodanese is a transferase enzyme that catalyses detoxification of cyanide. Cyanide is popular for its presence in a wide variety of food materials that are consumed by animals especially man. In this work, we investigated the partially purified rhodanese from cassava leaves in order to ascertain its suitability for cyanide detoxification in cassava tuber. Crude rhodanense was subjected to 80% (NH4)2SO4 precipitation, the percentage yield and purification fold obtained was 68% and 4 respectively. The degree of substrate preference was Na2S2O3>2-mercaptoethanol>ammonium persulphate>sodium metabisulfite>sodium sulfite. Apparent Km and Vmax were 18.2 mM and 13.9 RU/ml respectively. While that of Na2S2O3 were 10.9 mM and 5.8 RU/ml respectively. The substrate specificity Vmax/Km revealed that KCN was a better substrate. The optimum pH and temperature of rhodanese from cassava leaves were 6.0 and 40°C respectively. Result: The information provided by this study can be exploited for understanding cyanide distribution and detoxification of cassava.
Abstract: Introduction: Rhodanese is a transferase enzyme that catalyses detoxification of cyanide. Cyanide is popular for its presence in a wide variety of food materials that are consumed by animals especially man. In this work, we investigated the partially purified rhodanese from cassava leaves in order to ascertain its suitability for cyanide detoxifica...
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Acetylation of Cinnamic Acid and Evaluation of Antioxidant Activity of the Resultant Derivative
Olawale Hakeem Oladimeji,
Promise Omoridion Owere,
Paschal Chidera Anthony
Issue:
Volume 6, Issue 2, December 2021
Pages:
26-29
Received:
5 July 2021
Accepted:
27 July 2021
Published:
23 November 2021
Abstract: Background: Cinnamic acid is a white crystalline substance, chemically known as 2(E)-3-phenylprop-2-enoicacid or benzopropenoic acid. It is a mono-carboxylic acid naturally occurring in plants with low toxicity and serves as a flavoring agent in the food, pharmaceutical and perfumery industries. Objectives: The rising incidence of deleterious and harmful effects of free radicals in the human body leading to wide ranging disease conditions such as diabetes, cardiovascular ailments, cancers, hay-fever amongst many others necessitated this present study. Method: The acid was subjected to the acetylation reaction using acetic anhydride in acetic acid. In addition, the acid and the resultant derivative were screened for antioxidant activity using the DPPH (2, 2-diphenyl-1-picrylhydrazylhydrate) test. The comparison of the observed activities was also done. Results: The acetylation of the acid led to a derivative which has been identified to be cinnamyl acetate using the IR spectral technique. Both the cinnamic acid and acetyl derivative demonstrated remarkably significant antioxidant activity of IC50 at 0.18 and 0.16 µg/mL respectively which compare favorably with 0.12 µg/mL elicited by Vitamin C (a standard antioxidant drug). Conclusion: The obtained results indicate that acetylation slightly enhances the antioxidant activity of cinnamic acid.
Abstract: Background: Cinnamic acid is a white crystalline substance, chemically known as 2(E)-3-phenylprop-2-enoicacid or benzopropenoic acid. It is a mono-carboxylic acid naturally occurring in plants with low toxicity and serves as a flavoring agent in the food, pharmaceutical and perfumery industries. Objectives: The rising incidence of deleterious and h...
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