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The Molecular Identification Method for Purple Yam Cultivar Based on Genome RAPD Analysis

Received: 1 June 2016     Published: 5 July 2016
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Abstract

Based on the limited understanding of the former researchers on RAPD marker, numbers of cultivars of purple yam were utilized to evaluate the application of RAPD in cultivar identification of purple yam. The results showed that RAPD was a stable DNA marker technique, and it could be scientifically and easily used in cultivar identification purple yam. The RAPD fingerprints of purple yam crops, which were derived from primers with different number of nucleotides length, were different in band number, polymorphism and band clarity. Basically, RAPD primers with S1255 can show DNA fingerprint clearly in purple yam for the generation, while RAPD primers with S1255 is more suitable purple yam crops.

Published in Science Discovery (Volume 4, Issue 3)
DOI 10.11648/j.sd.20160403.11
Page(s) 161-164
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Purple Yam, RAPD, Cultivar Identification

References
[1] An N, Guo H B, KEW D. Genetic Variation in Rhizome Lotus (Nelumbo nucifera Gaertn. ssp. nucifera) Germplasms from China Assessed by RAPD Markers [J]. Agricultural Sciences in China, 2009, 8(1): 31~39.
[2] Yang RW, Zhou YH, Ding C B, et al Biologia Plantarum, Relationships among Leymus species assessed by RAPD markers [J]. Biologia Plantarum, 2008, 52(2): 237~241.
[3] Wang Y J, Lu JN. The research on RAPD genetic markers of grape seedlessness gene [J]. Journal of Northwest A & F University Na.t Sc.i Ed., 1996, 5(24): 10~20.
[4] Williams JG K, KubelikA R, Livak. J, et a.l DNA polymorphisms amplified by arbitrary primers are useful as genetic markers [J]. Nucl Acids Res, 1990, 18: 6531~6535.
[5] Lin KH, Lai Y C, Li H C. Genetic variation and its relationship to rootweight in the sweet potato as revealed by RAPD analysis [J]. Scientia Horticulturae, 2009, 120(1):2~7.
[6] Cai Y L, Cao D W, Zhao G F. Studies on genetic variation in cherry germplasm using RAPD analysis[J]. Scientia Horticulturae, 2007, 111(3): 248~254.
[7] 马艳芝,张玉星.梨种质资源遗传多样性研究中的RAPD技术引物筛选[J].中国农学通报,2009,25(11):30~33。
[8] 高道侠,文海涛,林励,等.江西酸橙不同栽培变种RAPD分析[J].安徽农业科学,2009,37(8):3457~3459。
[9] 李莉,彭建营,白瑞霞.中国枣属植物亲缘关系的RAPD分析[J].园艺学报,2009,36(4):475~480。
[10] 杨向晖,李平,刘成明,等.枇杷属植物及其近缘属植物亲缘关系的RAPD分析[J].果树学报,2009,26(1):55~59。
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  • APA Style

    Liu Pangyuan, Song Shuhui, Zhao Hong, He Weiming, Wang Wenqi, et al. (2016). The Molecular Identification Method for Purple Yam Cultivar Based on Genome RAPD Analysis. Science Discovery, 4(3), 161-164. https://doi.org/10.11648/j.sd.20160403.11

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    ACS Style

    Liu Pangyuan; Song Shuhui; Zhao Hong; He Weiming; Wang Wenqi, et al. The Molecular Identification Method for Purple Yam Cultivar Based on Genome RAPD Analysis. Sci. Discov. 2016, 4(3), 161-164. doi: 10.11648/j.sd.20160403.11

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    AMA Style

    Liu Pangyuan, Song Shuhui, Zhao Hong, He Weiming, Wang Wenqi, et al. The Molecular Identification Method for Purple Yam Cultivar Based on Genome RAPD Analysis. Sci Discov. 2016;4(3):161-164. doi: 10.11648/j.sd.20160403.11

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  • @article{10.11648/j.sd.20160403.11,
      author = {Liu Pangyuan and Song Shuhui and Zhao Hong and He Weiming and Wang Wenqi and Wang Huijie},
      title = {The Molecular Identification Method for Purple Yam Cultivar Based on Genome RAPD Analysis},
      journal = {Science Discovery},
      volume = {4},
      number = {3},
      pages = {161-164},
      doi = {10.11648/j.sd.20160403.11},
      url = {https://doi.org/10.11648/j.sd.20160403.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20160403.11},
      abstract = {Based on the limited understanding of the former researchers on RAPD marker, numbers of cultivars of purple yam were utilized to evaluate the application of RAPD in cultivar identification of purple yam. The results showed that RAPD was a stable DNA marker technique, and it could be scientifically and easily used in cultivar identification purple yam. The RAPD fingerprints of purple yam crops, which were derived from primers with different number of nucleotides length, were different in band number, polymorphism and band clarity. Basically, RAPD primers with S1255 can show DNA fingerprint clearly in purple yam for the generation, while RAPD primers with S1255 is more suitable purple yam crops.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - The Molecular Identification Method for Purple Yam Cultivar Based on Genome RAPD Analysis
    AU  - Liu Pangyuan
    AU  - Song Shuhui
    AU  - Zhao Hong
    AU  - He Weiming
    AU  - Wang Wenqi
    AU  - Wang Huijie
    Y1  - 2016/07/05
    PY  - 2016
    N1  - https://doi.org/10.11648/j.sd.20160403.11
    DO  - 10.11648/j.sd.20160403.11
    T2  - Science Discovery
    JF  - Science Discovery
    JO  - Science Discovery
    SP  - 161
    EP  - 164
    PB  - Science Publishing Group
    SN  - 2331-0650
    UR  - https://doi.org/10.11648/j.sd.20160403.11
    AB  - Based on the limited understanding of the former researchers on RAPD marker, numbers of cultivars of purple yam were utilized to evaluate the application of RAPD in cultivar identification of purple yam. The results showed that RAPD was a stable DNA marker technique, and it could be scientifically and easily used in cultivar identification purple yam. The RAPD fingerprints of purple yam crops, which were derived from primers with different number of nucleotides length, were different in band number, polymorphism and band clarity. Basically, RAPD primers with S1255 can show DNA fingerprint clearly in purple yam for the generation, while RAPD primers with S1255 is more suitable purple yam crops.
    VL  - 4
    IS  - 3
    ER  - 

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Author Information
  • National Engineering Research Center for Vegetables, Beijing, China

  • National Engineering Research Center for Vegetables, Beijing, China

  • National Engineering Research Center for Vegetables, Beijing, China

  • National Engineering Research Center for Vegetables, Beijing, China

  • National Engineering Research Center for Vegetables, Beijing, China

  • National Engineering Research Center for Vegetables, Beijing, China

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