Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect<sup>®</sup>) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters

Aksana Hancharuk; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Mayank Gangwar; Snehasis Jana

doi:doi:10.11648/j.ajls.20170503.11

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Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect^®) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters

Aksana Hancharuk, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Published in American Journal of Life Sciences (Volume 5, Issue 3)

Received: 27 March 2017 Accepted: 7 April 2017 Published: 8 May 2017

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Abstract

The current study aimed to evaluate the effect of Biofield Energy Healing (The Trivedi Effect^®) based herbomineral formulation on various skin health parameters. The test formulation consisted of minerals (zinc, selenate, and molybdenum), L-ascorbic acid, along with Centella asiatica extract, and tetrahydrocurcumin (THC). The test formulation and DMEM were divided into two parts, one part of each was treated with the Biofield Energy Treatment by Aksana Hancharuk and denoted as treated group, while other part was coded as the untreated groups. Skin parameters against various cell lines such as HFF-1, HaCaT and B16-F10 were examined with the combination of Biofield Treated (BT) and untreated test formulation (UT) with DMEM. MTT assay result showed that the test formulation was found to be safe and nontoxic at tested concentrations. Human fibroblast cell proliferation using BrdU method showed a significant increased cell proliferation by 91.51% and 201.14% in different Biofield Energy Treated groups. UT-DMEM + BT-Test formulation group showed an increased level of collagen by 64.58% (2.5 µg/mL) and 55.55% (1.25 µg/mL), respectively compared with the untreated group. Similarly, elastin synthesis was increased in UT-DMEM + BT-Test formulation group by 21.96% and 11.42% at concentration 10 and 5 µg/mL, respectively compared to the untreated group. However, hyaluronic acid level was increased by 14.02% and 6.57% at concentrations 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Moreover, inhibition of melanin synthesis was observed by 12.95% and 17.86% (0.125 µg/mL) in UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling activity in HFF-1 cells showed a notable cell viability in all the groups at various concentrations, i.e. in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation group by 15.68%, 36.47%, and 29.64%, respectively at 2.5 µg/mL compared with the untreated group. The significant wound healing activity was reported in scratch assay in HFF-1 and HaCaT cells due to the significantly higher cellular migration of fibroblast and keratinocytes. Therefore, Biofield Energy Treatment (The Trivedi Effect^®) based test formulation and cell medium might be a suitable approach for the development for herbal cosmetics and formulations as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating action. It can improve the overall skin health care against many skin disorders such as Eczema, diaper rash, seborrheic dermatitis, chickenpox, measles, warts, acne, hives, ringworm, Rosacea, psoriasis, skin cancer, etc.

Published in	American Journal of Life Sciences (Volume 5, Issue 3)
DOI	10.11648/j.ajls.20170503.11
Page(s)	75-85
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Consciousness Energy Healing Treatment, B16-F10, Extracellular Matrix, HaCaT, HFF-1, Hyaluronic Acid, Scratch Assay, Tetrahydrocurcumin

References

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Aksana Hancharuk, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. (2017). Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect®) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters. American Journal of Life Sciences, 5(3), 75-85. https://doi.org/10.11648/j.ajls.20170503.11

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ACS Style

Aksana Hancharuk; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak, et al. Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect®) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters. Am. J. Life Sci. 2017, 5(3), 75-85. doi: 10.11648/j.ajls.20170503.11

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AMA Style

Aksana Hancharuk, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect®) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters. Am J Life Sci. 2017;5(3):75-85. doi: 10.11648/j.ajls.20170503.11

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@article{10.11648/j.ajls.20170503.11,
  author = {Aksana Hancharuk and Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Mayank Gangwar and Snehasis Jana},
  title = {Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect®) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters},
  journal = {American Journal of Life Sciences},
  volume = {5},
  number = {3},
  pages = {75-85},
  doi = {10.11648/j.ajls.20170503.11},
  url = {https://doi.org/10.11648/j.ajls.20170503.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20170503.11},
  abstract = {The current study aimed to evaluate the effect of Biofield Energy Healing (The Trivedi Effect®) based herbomineral formulation on various skin health parameters. The test formulation consisted of minerals (zinc, selenate, and molybdenum), L-ascorbic acid, along with Centella asiatica extract, and tetrahydrocurcumin (THC). The test formulation and DMEM were divided into two parts, one part of each was treated with the Biofield Energy Treatment by Aksana Hancharuk and denoted as treated group, while other part was coded as the untreated groups. Skin parameters against various cell lines such as HFF-1, HaCaT and B16-F10 were examined with the combination of Biofield Treated (BT) and untreated test formulation (UT) with DMEM. MTT assay result showed that the test formulation was found to be safe and nontoxic at tested concentrations. Human fibroblast cell proliferation using BrdU method showed a significant increased cell proliferation by 91.51% and 201.14% in different Biofield Energy Treated groups. UT-DMEM + BT-Test formulation group showed an increased level of collagen by 64.58% (2.5 µg/mL) and 55.55% (1.25 µg/mL), respectively compared with the untreated group. Similarly, elastin synthesis was increased in UT-DMEM + BT-Test formulation group by 21.96% and 11.42% at concentration 10 and 5 µg/mL, respectively compared to the untreated group. However, hyaluronic acid level was increased by 14.02% and 6.57% at concentrations 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Moreover, inhibition of melanin synthesis was observed by 12.95% and 17.86% (0.125 µg/mL) in UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling activity in HFF-1 cells showed a notable cell viability in all the groups at various concentrations, i.e. in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation group by 15.68%, 36.47%, and 29.64%, respectively at 2.5 µg/mL compared with the untreated group. The significant wound healing activity was reported in scratch assay in HFF-1 and HaCaT cells due to the significantly higher cellular migration of fibroblast and keratinocytes. Therefore, Biofield Energy Treatment (The Trivedi Effect®) based test formulation and cell medium might be a suitable approach for the development for herbal cosmetics and formulations as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating action. It can improve the overall skin health care against many skin disorders such as Eczema, diaper rash, seborrheic dermatitis, chickenpox, measles, warts, acne, hives, ringworm, Rosacea, psoriasis, skin cancer, etc.},
 year = {2017}
}

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TY  - JOUR
T1  - Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect®) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters
AU  - Aksana Hancharuk
AU  - Mahendra Kumar Trivedi
AU  - Alice Branton
AU  - Dahryn Trivedi
AU  - Gopal Nayak
AU  - Mayank Gangwar
AU  - Snehasis Jana
Y1  - 2017/05/08
PY  - 2017
N1  - https://doi.org/10.11648/j.ajls.20170503.11
DO  - 10.11648/j.ajls.20170503.11
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 75
EP  - 85
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20170503.11
AB  - The current study aimed to evaluate the effect of Biofield Energy Healing (The Trivedi Effect®) based herbomineral formulation on various skin health parameters. The test formulation consisted of minerals (zinc, selenate, and molybdenum), L-ascorbic acid, along with Centella asiatica extract, and tetrahydrocurcumin (THC). The test formulation and DMEM were divided into two parts, one part of each was treated with the Biofield Energy Treatment by Aksana Hancharuk and denoted as treated group, while other part was coded as the untreated groups. Skin parameters against various cell lines such as HFF-1, HaCaT and B16-F10 were examined with the combination of Biofield Treated (BT) and untreated test formulation (UT) with DMEM. MTT assay result showed that the test formulation was found to be safe and nontoxic at tested concentrations. Human fibroblast cell proliferation using BrdU method showed a significant increased cell proliferation by 91.51% and 201.14% in different Biofield Energy Treated groups. UT-DMEM + BT-Test formulation group showed an increased level of collagen by 64.58% (2.5 µg/mL) and 55.55% (1.25 µg/mL), respectively compared with the untreated group. Similarly, elastin synthesis was increased in UT-DMEM + BT-Test formulation group by 21.96% and 11.42% at concentration 10 and 5 µg/mL, respectively compared to the untreated group. However, hyaluronic acid level was increased by 14.02% and 6.57% at concentrations 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Moreover, inhibition of melanin synthesis was observed by 12.95% and 17.86% (0.125 µg/mL) in UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling activity in HFF-1 cells showed a notable cell viability in all the groups at various concentrations, i.e. in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation group by 15.68%, 36.47%, and 29.64%, respectively at 2.5 µg/mL compared with the untreated group. The significant wound healing activity was reported in scratch assay in HFF-1 and HaCaT cells due to the significantly higher cellular migration of fibroblast and keratinocytes. Therefore, Biofield Energy Treatment (The Trivedi Effect®) based test formulation and cell medium might be a suitable approach for the development for herbal cosmetics and formulations as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating action. It can improve the overall skin health care against many skin disorders such as Eczema, diaper rash, seborrheic dermatitis, chickenpox, measles, warts, acne, hives, ringworm, Rosacea, psoriasis, skin cancer, etc.
VL  - 5
IS  - 3
ER  -

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Author Information

Aksana Hancharuk

Trivedi Global, Inc., Henderson, NV, USA
Mahendra Kumar Trivedi

Trivedi Global, Inc., Henderson, NV, USA
Alice Branton

Trivedi Global, Inc., Henderson, NV, USA
Dahryn Trivedi

Trivedi Global, Inc., Henderson, NV, USA
Gopal Nayak

Trivedi Global, Inc., Henderson, NV, USA
Mayank Gangwar

Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India
Snehasis Jana

Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India

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APA Style

Aksana Hancharuk, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. (2017). Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect®) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters. American Journal of Life Sciences, 5(3), 75-85. https://doi.org/10.11648/j.ajls.20170503.11

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ACS Style

Aksana Hancharuk; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak, et al. Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect®) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters. Am. J. Life Sci. 2017, 5(3), 75-85. doi: 10.11648/j.ajls.20170503.11

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AMA Style

Aksana Hancharuk, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect®) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters. Am J Life Sci. 2017;5(3):75-85. doi: 10.11648/j.ajls.20170503.11

Copy | Download

@article{10.11648/j.ajls.20170503.11,
  author = {Aksana Hancharuk and Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Mayank Gangwar and Snehasis Jana},
  title = {Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect®) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters},
  journal = {American Journal of Life Sciences},
  volume = {5},
  number = {3},
  pages = {75-85},
  doi = {10.11648/j.ajls.20170503.11},
  url = {https://doi.org/10.11648/j.ajls.20170503.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20170503.11},
  abstract = {The current study aimed to evaluate the effect of Biofield Energy Healing (The Trivedi Effect®) based herbomineral formulation on various skin health parameters. The test formulation consisted of minerals (zinc, selenate, and molybdenum), L-ascorbic acid, along with Centella asiatica extract, and tetrahydrocurcumin (THC). The test formulation and DMEM were divided into two parts, one part of each was treated with the Biofield Energy Treatment by Aksana Hancharuk and denoted as treated group, while other part was coded as the untreated groups. Skin parameters against various cell lines such as HFF-1, HaCaT and B16-F10 were examined with the combination of Biofield Treated (BT) and untreated test formulation (UT) with DMEM. MTT assay result showed that the test formulation was found to be safe and nontoxic at tested concentrations. Human fibroblast cell proliferation using BrdU method showed a significant increased cell proliferation by 91.51% and 201.14% in different Biofield Energy Treated groups. UT-DMEM + BT-Test formulation group showed an increased level of collagen by 64.58% (2.5 µg/mL) and 55.55% (1.25 µg/mL), respectively compared with the untreated group. Similarly, elastin synthesis was increased in UT-DMEM + BT-Test formulation group by 21.96% and 11.42% at concentration 10 and 5 µg/mL, respectively compared to the untreated group. However, hyaluronic acid level was increased by 14.02% and 6.57% at concentrations 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Moreover, inhibition of melanin synthesis was observed by 12.95% and 17.86% (0.125 µg/mL) in UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling activity in HFF-1 cells showed a notable cell viability in all the groups at various concentrations, i.e. in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation group by 15.68%, 36.47%, and 29.64%, respectively at 2.5 µg/mL compared with the untreated group. The significant wound healing activity was reported in scratch assay in HFF-1 and HaCaT cells due to the significantly higher cellular migration of fibroblast and keratinocytes. Therefore, Biofield Energy Treatment (The Trivedi Effect®) based test formulation and cell medium might be a suitable approach for the development for herbal cosmetics and formulations as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating action. It can improve the overall skin health care against many skin disorders such as Eczema, diaper rash, seborrheic dermatitis, chickenpox, measles, warts, acne, hives, ringworm, Rosacea, psoriasis, skin cancer, etc.},
 year = {2017}
}

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TY  - JOUR
T1  - Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect®) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters
AU  - Aksana Hancharuk
AU  - Mahendra Kumar Trivedi
AU  - Alice Branton
AU  - Dahryn Trivedi
AU  - Gopal Nayak
AU  - Mayank Gangwar
AU  - Snehasis Jana
Y1  - 2017/05/08
PY  - 2017
N1  - https://doi.org/10.11648/j.ajls.20170503.11
DO  - 10.11648/j.ajls.20170503.11
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 75
EP  - 85
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20170503.11
AB  - The current study aimed to evaluate the effect of Biofield Energy Healing (The Trivedi Effect®) based herbomineral formulation on various skin health parameters. The test formulation consisted of minerals (zinc, selenate, and molybdenum), L-ascorbic acid, along with Centella asiatica extract, and tetrahydrocurcumin (THC). The test formulation and DMEM were divided into two parts, one part of each was treated with the Biofield Energy Treatment by Aksana Hancharuk and denoted as treated group, while other part was coded as the untreated groups. Skin parameters against various cell lines such as HFF-1, HaCaT and B16-F10 were examined with the combination of Biofield Treated (BT) and untreated test formulation (UT) with DMEM. MTT assay result showed that the test formulation was found to be safe and nontoxic at tested concentrations. Human fibroblast cell proliferation using BrdU method showed a significant increased cell proliferation by 91.51% and 201.14% in different Biofield Energy Treated groups. UT-DMEM + BT-Test formulation group showed an increased level of collagen by 64.58% (2.5 µg/mL) and 55.55% (1.25 µg/mL), respectively compared with the untreated group. Similarly, elastin synthesis was increased in UT-DMEM + BT-Test formulation group by 21.96% and 11.42% at concentration 10 and 5 µg/mL, respectively compared to the untreated group. However, hyaluronic acid level was increased by 14.02% and 6.57% at concentrations 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Moreover, inhibition of melanin synthesis was observed by 12.95% and 17.86% (0.125 µg/mL) in UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling activity in HFF-1 cells showed a notable cell viability in all the groups at various concentrations, i.e. in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation group by 15.68%, 36.47%, and 29.64%, respectively at 2.5 µg/mL compared with the untreated group. The significant wound healing activity was reported in scratch assay in HFF-1 and HaCaT cells due to the significantly higher cellular migration of fibroblast and keratinocytes. Therefore, Biofield Energy Treatment (The Trivedi Effect®) based test formulation and cell medium might be a suitable approach for the development for herbal cosmetics and formulations as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating action. It can improve the overall skin health care against many skin disorders such as Eczema, diaper rash, seborrheic dermatitis, chickenpox, measles, warts, acne, hives, ringworm, Rosacea, psoriasis, skin cancer, etc.
VL  - 5
IS  - 3
ER  -

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