To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells

Karen Poon; Tse Chiu Chung; Chang Xu; Ruihua Wang

doi:doi:10.11648/j.ajls.20140203.17

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To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells

Karen Poon, Tse Chiu Chung, Chang Xu, Ruihua Wang

Published in American Journal of Life Sciences (Volume 2, Issue 3)

Received: 5 April 2014 Accepted: 26 April 2014 Published: 30 June 2014

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Abstract

Proton leak has been implicated in various chronic diseases like diabetes and cancer. In this study, current from intact cells, including mice liver cells, pig blood cells and human breast cancer cell MCF-7 were measured by microbial fuel cells (MFC). Positive current change in normal liver cells were induced by either 2,4-dinitrophenol (DNP) or Piceatonnol. The effect of DNP in enhancing the proton conductivity would increase the degree of positive current change, while Piceatonnol in improving the mitochondria membrane potential would support the sustainability of the positive current change with time. Piceatonnol was found to be more effective in inducing positive current change in cancer cells than in liver cells. The higher effectiveness of Piceatonnol to cancer cells would be explained by the high proton leak condition of the cells, and so increased the current production. Little positive current change could be induced in red blood cell by either DNP or Piceatonnol. Results supported the hypothesis of the high mitochondria membrane potential to support the positive current change in cells with time, while the proton conductivity determined the degree of positive current change. The condition of proton leak of cells seemed to be the limiting factor for the positive current change in cells.

Published in	American Journal of Life Sciences (Volume 2, Issue 3)
DOI	10.11648/j.ajls.20140203.17
Page(s)	176-181
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Proton Leak, Electron Leak, Microbial Fuel Cells, 2,4-Dinitrophenol, Piceatonnol

References

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APA Style

Karen Poon, Tse Chiu Chung, Chang Xu, Ruihua Wang. (2014). To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells. American Journal of Life Sciences, 2(3), 176-181. https://doi.org/10.11648/j.ajls.20140203.17

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ACS Style

Karen Poon; Tse Chiu Chung; Chang Xu; Ruihua Wang. To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells. Am. J. Life Sci. 2014, 2(3), 176-181. doi: 10.11648/j.ajls.20140203.17

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AMA Style

Karen Poon, Tse Chiu Chung, Chang Xu, Ruihua Wang. To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells. Am J Life Sci. 2014;2(3):176-181. doi: 10.11648/j.ajls.20140203.17

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@article{10.11648/j.ajls.20140203.17,
  author = {Karen Poon and Tse Chiu Chung and Chang Xu and Ruihua Wang},
  title = {To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {3},
  pages = {176-181},
  doi = {10.11648/j.ajls.20140203.17},
  url = {https://doi.org/10.11648/j.ajls.20140203.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140203.17},
  abstract = {Proton leak has been implicated in various chronic diseases like diabetes and cancer. In this study, current from intact cells, including mice liver cells, pig blood cells and human breast cancer cell MCF-7 were measured by microbial fuel cells (MFC). Positive current change in normal liver cells were induced by either 2,4-dinitrophenol (DNP) or Piceatonnol. The effect of DNP in enhancing the proton conductivity would increase the degree of positive current change, while Piceatonnol in improving the mitochondria membrane potential would support the sustainability of the positive current change with time. Piceatonnol was found to be more effective in inducing positive current change in cancer cells than in liver cells. The higher effectiveness of Piceatonnol to cancer cells would be explained by the high proton leak condition of the cells, and so increased the current production. Little positive current change could be induced in red blood cell by either DNP or Piceatonnol. Results supported the hypothesis of the high mitochondria membrane potential to support the positive current change in cells with time, while the proton conductivity determined the degree of positive current change. The condition of proton leak of cells seemed to be the limiting factor for the positive current change in cells.},
 year = {2014}
}

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TY  - JOUR
T1  - To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells
AU  - Karen Poon
AU  - Tse Chiu Chung
AU  - Chang Xu
AU  - Ruihua Wang
Y1  - 2014/06/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ajls.20140203.17
DO  - 10.11648/j.ajls.20140203.17
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 176
EP  - 181
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20140203.17
AB  - Proton leak has been implicated in various chronic diseases like diabetes and cancer. In this study, current from intact cells, including mice liver cells, pig blood cells and human breast cancer cell MCF-7 were measured by microbial fuel cells (MFC). Positive current change in normal liver cells were induced by either 2,4-dinitrophenol (DNP) or Piceatonnol. The effect of DNP in enhancing the proton conductivity would increase the degree of positive current change, while Piceatonnol in improving the mitochondria membrane potential would support the sustainability of the positive current change with time. Piceatonnol was found to be more effective in inducing positive current change in cancer cells than in liver cells. The higher effectiveness of Piceatonnol to cancer cells would be explained by the high proton leak condition of the cells, and so increased the current production. Little positive current change could be induced in red blood cell by either DNP or Piceatonnol. Results supported the hypothesis of the high mitochondria membrane potential to support the positive current change in cells with time, while the proton conductivity determined the degree of positive current change. The condition of proton leak of cells seemed to be the limiting factor for the positive current change in cells.
VL  - 2
IS  - 3
ER  -

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Author Information

Karen Poon

Program of Food Science and Technology, Division of Science and Technology, BNU-HKBU United International College, 28 Jinfeng Road, Tangjiawan, Zhuhai, Guangdong, China
Tse Chiu Chung

Program of Food Science and Technology, Division of Science and Technology, BNU-HKBU United International College, 28 Jinfeng Road, Tangjiawan, Zhuhai, Guangdong, China
Chang Xu

Program of Food Science and Technology, Division of Science and Technology, BNU-HKBU United International College, 28 Jinfeng Road, Tangjiawan, Zhuhai, Guangdong, China
Ruihua Wang

Department of Gastroenterology, Shanghai Jiao Tong University affiliated Sixth People’s Hospital South Campus, 6600 Nanfeng Road, Fengxian District, Shanghai, China

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APA Style

Karen Poon, Tse Chiu Chung, Chang Xu, Ruihua Wang. (2014). To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells. American Journal of Life Sciences, 2(3), 176-181. https://doi.org/10.11648/j.ajls.20140203.17

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ACS Style

Karen Poon; Tse Chiu Chung; Chang Xu; Ruihua Wang. To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells. Am. J. Life Sci. 2014, 2(3), 176-181. doi: 10.11648/j.ajls.20140203.17

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AMA Style

Karen Poon, Tse Chiu Chung, Chang Xu, Ruihua Wang. To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells. Am J Life Sci. 2014;2(3):176-181. doi: 10.11648/j.ajls.20140203.17

Copy | Download

@article{10.11648/j.ajls.20140203.17,
  author = {Karen Poon and Tse Chiu Chung and Chang Xu and Ruihua Wang},
  title = {To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {3},
  pages = {176-181},
  doi = {10.11648/j.ajls.20140203.17},
  url = {https://doi.org/10.11648/j.ajls.20140203.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140203.17},
  abstract = {Proton leak has been implicated in various chronic diseases like diabetes and cancer. In this study, current from intact cells, including mice liver cells, pig blood cells and human breast cancer cell MCF-7 were measured by microbial fuel cells (MFC). Positive current change in normal liver cells were induced by either 2,4-dinitrophenol (DNP) or Piceatonnol. The effect of DNP in enhancing the proton conductivity would increase the degree of positive current change, while Piceatonnol in improving the mitochondria membrane potential would support the sustainability of the positive current change with time. Piceatonnol was found to be more effective in inducing positive current change in cancer cells than in liver cells. The higher effectiveness of Piceatonnol to cancer cells would be explained by the high proton leak condition of the cells, and so increased the current production. Little positive current change could be induced in red blood cell by either DNP or Piceatonnol. Results supported the hypothesis of the high mitochondria membrane potential to support the positive current change in cells with time, while the proton conductivity determined the degree of positive current change. The condition of proton leak of cells seemed to be the limiting factor for the positive current change in cells.},
 year = {2014}
}

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TY  - JOUR
T1  - To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells
AU  - Karen Poon
AU  - Tse Chiu Chung
AU  - Chang Xu
AU  - Ruihua Wang
Y1  - 2014/06/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ajls.20140203.17
DO  - 10.11648/j.ajls.20140203.17
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 176
EP  - 181
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20140203.17
AB  - Proton leak has been implicated in various chronic diseases like diabetes and cancer. In this study, current from intact cells, including mice liver cells, pig blood cells and human breast cancer cell MCF-7 were measured by microbial fuel cells (MFC). Positive current change in normal liver cells were induced by either 2,4-dinitrophenol (DNP) or Piceatonnol. The effect of DNP in enhancing the proton conductivity would increase the degree of positive current change, while Piceatonnol in improving the mitochondria membrane potential would support the sustainability of the positive current change with time. Piceatonnol was found to be more effective in inducing positive current change in cancer cells than in liver cells. The higher effectiveness of Piceatonnol to cancer cells would be explained by the high proton leak condition of the cells, and so increased the current production. Little positive current change could be induced in red blood cell by either DNP or Piceatonnol. Results supported the hypothesis of the high mitochondria membrane potential to support the positive current change in cells with time, while the proton conductivity determined the degree of positive current change. The condition of proton leak of cells seemed to be the limiting factor for the positive current change in cells.
VL  - 2
IS  - 3
ER  -

Copy | Download