Brown adipose tissues (BAT) have special active functions including thermogenesis, anti-obesity, and anti-diabetic properties. Researchers have shown the immune roles of BAT in relation immune depression in deep hypothermia and lymphocyte accumulation in the spleen as detected in adipectomized rats. In this study we aimed to clarify immune functions of hibernating marmot BAT which continue to be used in pancreatitis treatment in Mongolia. In this study, 10 marmots were selected. The subject pool was limited license are needed for marmot use. Disengaged BAT was located between subcapularis and serratus anterior muscles. It was inserted into 96% of alcohol and 10% formalin for fixation. Histology examination was used with standard protocol of hematoxylin and eosin staining protocol. Fluorescence-activated cell sorting method (Apogee Universal-50, England) was used to count the CD4+ and CD8+ T cells in the BAT of mice. The study selected BALB/c mode, 20 male mice 80-90 days-old. Marmots are not experimental animals therefore reagents of tissue cell count were limited. The BAT (marmot) histology observation showed connective tissue in the lobules reached by blood vessel branches and detected brown fat tissue surrounding follicle which included vessels with blood cells. Regarding tissue cell count results, the BAT detected a total of 24827/μL lymphocytes, which included 2056/μL (5.50%) CD4+ T cells and 991/μL (6.71%) CD8+ T cells, respectively. This study’s results suggest the brown fat is may be an immune organ as shown by the lymphoid tissue follicle and lymphocyte subsets in the BAT.
| Published in | American Journal of Clinical and Experimental Medicine (Volume 2, Issue 1) |
| DOI | 10.11648/j.ajcem.20140201.12 |
| Page(s) | 4-8 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Brown Adipose Tissue, Immune Cell, CD4+, CD8+
| [1] | Gessner K. History of Animals, "Conradi Gesneri medici Tiguine Historiae Animalium Lib I de Quadripedibus uiuparis" Book 1 of Four-Legged Uniparous 1551, pp.840-844. |
| [2] | Carlos jinqueira L, "Basis histology" 1996, pp. 129-131. |
| [3] | Orava J, Nuutila P, Oikonen V, Noponen T, Virtanen KA, "Human brown adipose tissue glucose uptake but not perfusion is stimulated by insulin" [ICO 2010 congress abstract]. Obesity |
| [4] | B.D. Jankovic, AlenkaJanezic and Ljiljana Popeskovic, "Brown adipose tissue and immunity. Effect of neonatal adipectomy on humoral and cellular immune reactions in the rat" Immunology 1975, 28:597-609. |
| [5] | Petric. J, "Contribution a la serologie des mammiferes" Publ.Fac.Med.Brno R.C.S 1992, 1, 247. |
| [6] | Schmidt. J. P, "Response of hibernating animals to physical, parasites and infectious agents" Mammalian Hibernation volume 3 (By K. C. fisher, A.R. Dawe, C.P.Lyman, E.Schonbaum and F.E.South, Jr), 1967, pp. 421. Oliver & Boyd, Edinburgh |
| [7] | Jancovic, B. D., Popeskovic, L., Janezic, A. and Lukic, M.L, "Brown adipose tissue: effect on immune reactions in the rat" Naturwissenschaften, 1974; pp.61,36. |
| [8] | Educational guide, Special Stains and H&E, Second Edition. George L.Kumar, John A. Kierman 2010. |
| [9] | Feldmanm HM, Golozoubova V, Cannon B, Nedergaard J, "UCP1 ablation induces obesity and abolishes diet-indused thermogenesis in mice exempt from thermal stress by living at thermoneutrality" Cell Metab 2009; 9:203-209. |
| [10] | Cannon B, Nedergaard J, "Thermogenesis challenges the adipostat hypothesis for body-weight control" Proc Nutr Soc 2009; 68:401-407. |
| [11] | Cederberg A, Gronning LM, Ahren B, Tasken K, Carlsson P, Enerback S, "FOXC2 is a winged helix gene that counteracts obesity, hypertriglyceridemia, and diet-induced insulin resistance" Cell 2001; 106:563–573. |
| [12] | Hamann A, Flier JS, Lowell BB, "Decreased brown fat markedly enhances susceptibility to diet-induced obesity, diabetes, and hyperlipidemia" Endocrinology 1996; 137:21–29 |
| [13] | Cannon B, Nedergaard J. Brown adipose tissue: function and physiological significance. Physiol Rev. 2004; 84:277–359. |
| [14] | Г.Пүрэвдорж "Хүн ба зарим хөхтөн амьтны бор өөхний бүтэц, тосны хүчлийн агууламж, химийн бүрдлийн онцлогийг судлах асуудалд" докторын ажил 2005 он хуудас 55-60 |
| [15] | Leonard Napolitano and Don Fawcett, "The fine structure of brown adipose tissue in the newborn mouse and rat" J Biophys Biochem Cytol. 1958; 25:685-692. |
| [16] | Tovi F, Mares AJ, The aberrant cervical thymus: Embriology, pathology, and clinical implication. Am J Surgery, 1978; 136:631-637. |
| [17] | Ushiyama I, Kouno A, Yamamoto Y, Nishimura A, Ito, et al, "Hassall’s corpuscle fumction as the secretory cell groups" Forensic Pathology 1997; 3: 111-117. |
| [18] | Romagnani P, Annunzaito R, Mavilia C, Lasagni L, et al, "High CD30 ligand expression by epithelial cells and Hassall’s corpuscles in the medulla of human thymus ", Blood 1998; 91:3323-3332. |
| [19] | Nishio H, Matsukui K, Tamura A, Suzuki K, Immunolocalization of the mitogen-activated protein kinase signaling pathway in Hassall’s corpuscles of the human thymus. Acta Histomech 2001; 103: 89-98. |
| [20] | Watanabe N, Wang YH, Lee HK, Ito T, Wang YH, et al, Hassall’s Corpuscles instruct dendritic cells to induce CD4+CD25+ regulatory T cells in human thymus. Nature 2005; 436:1181-1185. |
| [21] | "Histochemical and morphological characteristics of the Hassall’s corpuscles. The stress affects involution of the ectopic intra-thyroidal and the normal position thymus and morphological changes of the Hassall’s corpuscles" J Forensic Res 2012; 3:9. |
| [22] | Dagdanbazar B, Dugarsuren C, "Situation study of marmot "Khun makh" Presentation. Ulaanbaatar city,1990, pp.36-40. (in Mongolian) |
| [23] | Jancovic, B. D., Popeskovic, L., Janezic, A. and Lukic, M.L, "Brown adipose tissue: effect on immune reactions in the rat" Naturwissenschaften, 1974; pp.61,36. |
| [24] | Dagdanbazar D, "Role of publication for development of modern anatomy of Mongolian doctors" Dissertation –UB; 2002, 126-133. (In Mongolian) |
| [25] | Khongorzul B, "Some treatment effects in derived arthritis mouse model by roentgen analyses" dissertation of master science. UB 2013, pp. 59-60 (in Mongolian). |
APA Style
NYAMDORJ Dagdanbazar, DAGDANBAZAR Bodi, UURTUYA Shuumarjav, AMGALANBAATAR Dorjkhuu, MUNKHTULGA Lkhagvasuren, et al. (2014). Experimental Study Detection of CD4+ and CD8+ T Cells in the Brown Adipose Tissue. American Journal of Clinical and Experimental Medicine, 2(1), 4-8. https://doi.org/10.11648/j.ajcem.20140201.12
ACS Style
NYAMDORJ Dagdanbazar; DAGDANBAZAR Bodi; UURTUYA Shuumarjav; AMGALANBAATAR Dorjkhuu; MUNKHTULGA Lkhagvasuren, et al. Experimental Study Detection of CD4+ and CD8+ T Cells in the Brown Adipose Tissue. Am. J. Clin. Exp. Med. 2014, 2(1), 4-8. doi: 10.11648/j.ajcem.20140201.12
AMA Style
NYAMDORJ Dagdanbazar, DAGDANBAZAR Bodi, UURTUYA Shuumarjav, AMGALANBAATAR Dorjkhuu, MUNKHTULGA Lkhagvasuren, et al. Experimental Study Detection of CD4+ and CD8+ T Cells in the Brown Adipose Tissue. Am J Clin Exp Med. 2014;2(1):4-8. doi: 10.11648/j.ajcem.20140201.12
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ajcem.20140201.12,
author = {NYAMDORJ Dagdanbazar and DAGDANBAZAR Bodi and UURTUYA Shuumarjav and AMGALANBAATAR Dorjkhuu and MUNKHTULGA Lkhagvasuren and ENEBISH Sundui},
title = {Experimental Study Detection of CD4+ and CD8+ T Cells in the Brown Adipose Tissue},
journal = {American Journal of Clinical and Experimental Medicine},
volume = {2},
number = {1},
pages = {4-8},
doi = {10.11648/j.ajcem.20140201.12},
url = {https://doi.org/10.11648/j.ajcem.20140201.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20140201.12},
abstract = {Brown adipose tissues (BAT) have special active functions including thermogenesis, anti-obesity, and anti-diabetic properties. Researchers have shown the immune roles of BAT in relation immune depression in deep hypothermia and lymphocyte accumulation in the spleen as detected in adipectomized rats. In this study we aimed to clarify immune functions of hibernating marmot BAT which continue to be used in pancreatitis treatment in Mongolia. In this study, 10 marmots were selected. The subject pool was limited license are needed for marmot use. Disengaged BAT was located between subcapularis and serratus anterior muscles. It was inserted into 96% of alcohol and 10% formalin for fixation. Histology examination was used with standard protocol of hematoxylin and eosin staining protocol. Fluorescence-activated cell sorting method (Apogee Universal-50, England) was used to count the CD4+ and CD8+ T cells in the BAT of mice. The study selected BALB/c mode, 20 male mice 80-90 days-old. Marmots are not experimental animals therefore reagents of tissue cell count were limited. The BAT (marmot) histology observation showed connective tissue in the lobules reached by blood vessel branches and detected brown fat tissue surrounding follicle which included vessels with blood cells. Regarding tissue cell count results, the BAT detected a total of 24827/μL lymphocytes, which included 2056/μL (5.50%) CD4+ T cells and 991/μL (6.71%) CD8+ T cells, respectively. This study’s results suggest the brown fat is may be an immune organ as shown by the lymphoid tissue follicle and lymphocyte subsets in the BAT.},
year = {2014}
}
TY - JOUR T1 - Experimental Study Detection of CD4+ and CD8+ T Cells in the Brown Adipose Tissue AU - NYAMDORJ Dagdanbazar AU - DAGDANBAZAR Bodi AU - UURTUYA Shuumarjav AU - AMGALANBAATAR Dorjkhuu AU - MUNKHTULGA Lkhagvasuren AU - ENEBISH Sundui Y1 - 2014/02/20 PY - 2014 N1 - https://doi.org/10.11648/j.ajcem.20140201.12 DO - 10.11648/j.ajcem.20140201.12 T2 - American Journal of Clinical and Experimental Medicine JF - American Journal of Clinical and Experimental Medicine JO - American Journal of Clinical and Experimental Medicine SP - 4 EP - 8 PB - Science Publishing Group SN - 2330-8133 UR - https://doi.org/10.11648/j.ajcem.20140201.12 AB - Brown adipose tissues (BAT) have special active functions including thermogenesis, anti-obesity, and anti-diabetic properties. Researchers have shown the immune roles of BAT in relation immune depression in deep hypothermia and lymphocyte accumulation in the spleen as detected in adipectomized rats. In this study we aimed to clarify immune functions of hibernating marmot BAT which continue to be used in pancreatitis treatment in Mongolia. In this study, 10 marmots were selected. The subject pool was limited license are needed for marmot use. Disengaged BAT was located between subcapularis and serratus anterior muscles. It was inserted into 96% of alcohol and 10% formalin for fixation. Histology examination was used with standard protocol of hematoxylin and eosin staining protocol. Fluorescence-activated cell sorting method (Apogee Universal-50, England) was used to count the CD4+ and CD8+ T cells in the BAT of mice. The study selected BALB/c mode, 20 male mice 80-90 days-old. Marmots are not experimental animals therefore reagents of tissue cell count were limited. The BAT (marmot) histology observation showed connective tissue in the lobules reached by blood vessel branches and detected brown fat tissue surrounding follicle which included vessels with blood cells. Regarding tissue cell count results, the BAT detected a total of 24827/μL lymphocytes, which included 2056/μL (5.50%) CD4+ T cells and 991/μL (6.71%) CD8+ T cells, respectively. This study’s results suggest the brown fat is may be an immune organ as shown by the lymphoid tissue follicle and lymphocyte subsets in the BAT. VL - 2 IS - 1 ER -
Email: