The incidence of fungal leaf spot diseases on mango (Mangifera indica) in Oda Bultum University Mango farm and application of fungicide and a biological control measure was investigated. In addition microbial biocontrol and fungicide control against C. gloeosporioides developed for mangoes has been less reported in Ethiopia. Common mango disease control mechanisms in Ethiopia are utilization of pesticides. However, pesticides are overused and misused. As a result there is an urgent need to reduce the use of synthetic chemicals. Biological control offers an alternative to the use of pesticides. Invitro evaluation and testing of T. harzianum showed maximum mycelial growth inhibition (74.4% and 73.5%) on C. gloeosporioides isolates 1 and 3 respectively. The minimum percent of mycelial growth inhibition (71.5%) of T. harzianum was observed on isolate 1. Pseudomonas fluorescens showed maximum mycelial growth inhibition on isolate 2 (74.1%), followed by isolate 1 (70.4%). Least percent of mycelial growth inhibition by P. fluorescens was observed on isolate 3 (69%). B. subtilis showed maximum mycelial growth inhibition on isolate 3 (56.1%), followed by isolate 1 (51.5%). Least percent of mycelial growth inhibition by B. subtilis was observed on isolate 2 (48.6%). In vitro evaluation of fungicides against the three isolated test pathogen revealed that at 1000 PPM, all fungicides showed highest percentage inhibition (72.8% to 90.14%). The highest percentage inhibition was observed at the concentration of1000 PPM on isolates 2 (90.14%) and 3 (88.13%) and the lowest percentage inhibition was observed at the concentration of 200 PPM on isolates 1 (71.46%) and 3 (77.18%). The highest percentage mycelial growth inhibition by Sancozeb 80% WP on the isolates was 90.14% at a concentration of 1000PPM, whereas the lowest percentage mycelia growth inhibition of 83.06% was recorded at 200PPM. The highest percentage mycelial growth inhibition by Ridomil on the isolates was (83.16%) at 1000 PPM and the lowest percentage mycelial growth inhibition was recorded on isolate 1 (72.83%) at 200 PPM. Among the two fungicides Sancozeb was the most effective fungicide to inhibit the growth of the isolates with 87.98-90.14% inhibition.
| Published in | American Journal of Biomedical and Life Sciences (Volume 6, Issue 6) |
| DOI | 10.11648/j.ajbls.20180606.12 |
| Page(s) | 118-126 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Biocontrol, Colletotrichum gloeosporioides, Epiphytes, Mango, Leaf Spot, in Vitro, Mycelial Growth, Microbial Antagonists
| [1] | Panuwet P., Siriwong W., Prapamontol T., Ryan P. B., Fiedler N., Robson M. G., Barr D. B. (2012). Agricultural pesticide management in Thailand: Situation and population health risk. Environmental Science and Policy 17 (1): 72–81. |
| [2] | Panuwet P., Prapamontol T., Chantara S., Thavornyuthikarn P., Montesano M. A., Whitehead Jr R. D., Barr D. B. (2008). Concentrations of urinary pesticide metabolites in small-scale farmers in Chiang Mai Province, Thailand. Science of the Total Environment 407 (1): 655–668. |
| [3] | Holb I. J. (2009). Fungal disease management in environmentally friendly apple production – A Review. p. 219–292. In: “Climate Change, Intercropping, Pest Control and Beneficial Microorganisms” (Lichtfouse E., ed.). Springer, The Netherlands, 514 pp. |
| [4] | Singh L. B. (1960): The Mango, Botany, Cultivation and Utilization. London, Leonard Hills Ltd. |
| [5] | OAE. (2012). Office of Agricultural Economics of Thailand, Agricultural Statistics. Available on: http://www.oae.go.th/oae_report/export_import/export.php [Accessed: January 9, 2016]. |
| [6] | Kongtragoul P., Nalumpang S., Miyamoto Y., Izumi Y. and Kazuya Akimitsu K. (2011). Mutation at Codon 198 of Tub2 Gene for Carbendazim Resistance in Colletotrichum Gloeosporioides Causing Mango Anthracnose in Thailand. J. Plant Protection. 15 (4) 377–384. |
| [7] | Okigbo R. N. (2001): Occurrence, pathogenicity and survival of Macrophoma mangiferae in leaves, bran¬ches and stems of mango (Mangifera indica L.). Plant Protect. Sci., 37: 138–144. |
| [8] | Verma K. S., Cheema S. S., Kang M. S., and Sharma A. K. (1991): Hitherto unrecorded disease problems of mango from Punjab. Plant Dis. Res., 6: 141–142. |
| [9] | Janisiewicz W. J., Conway W. S. (2010). Combining biological control with physical and chemical treatments to control fruit decay after harvest. Stewart Postharvest Review 6 (1): 1–16. |
| [10] | Janisiewicz W. J., Jurick Ii W. M., Vico I., Peter K. A., Buyer J. S. (2013). Culturable bacteria from plum fruit surfaces and their potential for controlling brown rot after harvest. Postharvest Biology and Technology 76: 145–151. |
| [11] | Amin A., Khan M. A., Ehsanullah M., Haroon U., Azam S. M., Hameed A. 2012. Production of peptide antibiotics by Bacillus sp. GU 057 indigenously isolated from saline soil. Brazilian Journal of Microbiology 43: 1340–1346. |
| [12] | M. I., Ecoco H., and Okigbo R. N. (2001): Effect of isolates of Trichoderma species on Fusarium oxyspo¬rum f.sp. Lycopersici in vitro. Nigerian J. Microbiol., 15: 175–150. |
| [13] | Okigbo R. N. (2002): Mycoflora of tuber surface of white yam (Dioscorea rotundata Poir) and post harvest control of pathogens with Bacillus subtilis. Mycopathologia, 156: 81–85. |
| [14] | Arzanlou M., Bakhshi M., Karimi K., Torbati M. 2015. Multigene phylogeny reveals three new records of Colletotrichum spp. and several new host records for the mycobiota of Iran. Journal of Plant Protection Research 55 (2): 198–211. |
| [15] | Valero M., Garcia-Martinez S., Giner M. J., Alonso A., Ruiz J. J. 2010. Benomyl sensitivity assays and species-specific PCR reactions highlight association of two Colletotrichum gloeosporioides types and C. acutatum with rumple disease on Primofiori lemons. European Journal of Plant Pathology 127 (3): 399–405. |
| [16] | Peeran M. F., Krishnan N., Thangamani P. R., Gandhi K., Thiruvengadam R., Kuppusamy P. 2014. Development and evaluation of water-in-oil formulation of Pseudomonas fluorescens (FP7) against Colletotrichum musae incitant of anthracnose disease in banana. European Journal of Plant Pathology 138 (1): 167–180. |
| [17] | Mochizuki M., Yamamoto S., Aoki Y., Suzuki S. (2012). Isolation and characterization of Bacillus amyloliquefaciens S13-3 as a biological control agent for anthracnose caused by Colletotrichum gloeosporioides. Biocontrol Science and Technology 22 (6): 697–709. |
| [18] | Lima J. R., Gondim D. M. F., Oliveira J. T. A., Oliveira F. S. A., Gonçalves L. R. B., Viana F. M. P. (2013). Use of killer yeast in the management of postharvest papaya anthracnose. Postharvest Biology and Technology 83 (1): 58–64. |
| [19] | Admasu W., Sahile S., Kibret M. 2014. Assessment of potential antagonists for anthracnose (Colletotrichum gloeosporioides) disease of mango (Mangifera indica L.) in North Western Ethiopia (Pawe). Archives of Phytopathology and Plant Protection 47 (18): 2176–2186. |
| [20] | Khiyami M. A., Omar M. R., Abd-Elsalam K. A., El-Hady Aly A. A. 2014. Bacillus-based biological control of cotton seedling disease complex. Journal of Plant Protection Research 54 (4): 340–348. |
| [21] | Sharma R. R., Singh D., Singh R. 2009. Biological control of postharvest diseases of fruits and vegetables by microbial antagonists: A Review. Biological Control 50 (3): 205–221. |
| [22] | Ruangwong O. U., Chang C. I., Lamine S. A., Liang W. J. 2012. Identification of antifungal compound produced by Bacillus subtilis LB5 with ability to control anthracnose disease caused by Colletotrichum gloeosporioides. African Journal of Microbiology Research 6 (16): 3732–3738. |
| [23] | Alamri S., Hashem M., Mostafa Y. S. (2012). In vitro and in vivo biocontrol of soil-borne phytopathogenic fungi by certain bioagents and their possible mode of action. Biocontrol Science17 (4): 155–167. |
| [24] | Tesfaye Alemu and Kapoor, I. J. (2004). In Vitro evaluation of Trichoderma and Gliocladium spp. against Botrytis corm rot (Botrytis gladiolorum) of Gladiolus. Pest Mgt. J. Ethiopia 8: 97-103. |
| [25] | Rao, N. S. S. (2003). Methods used in soil Microbiological studies. Soil Microbiology. 4th edn. Oxford and IBH Publishing Co. Pvt. Ltd. New Delhi. Pp: 61-72. |
| [26] | Rangajaran, S., Saleena, L. M., Vasudevan, P. and Nair, S. (2003). Biological suppression of rice diseases by Pseudomonas spp. under saline soil conditions. Plant Soil 251: 73–82. |
| [27] | Riungu, G. M., Muthorni, J. W., Narla, R. D., Wagacha, J. M. and Gathumbi, J. K. (2008). Management of Fusarium head blight of wheat and deoxynivalenol accumulation using antagonistic microorganisms. Plant Pathol. J. 7: 13-19. |
| [28] | Nene, Y. L and Thapliyal, P. N. (1993). Fungicides in Plant Disease Control. Oxford and IBH Publishing CO. PVT. LTD. New Delhi, India. Pp.579. |
| [29] | Barnett, H. L. and Hunter, B. B. (1960). Illustrated Genera of Imperfect Fungi. 2nd edn. Burgess publishing company, Morgantown, West Virginia. Pp. 71. |
| [30] | Meena, B. S. (2005). Morphological and Molecular Variability of Rice Blast Pathogen Pyricularia grisea (Cooke) Sacc. M. Sc. Thesis. Dharwad University of Agricultural Sciences, Dharwad. |
| [31] | Gordon R. E., Haynes W. C., Hor-Nay Pang C., (1973): The Genus Bacillus. Agriculture Handbook No. 427. Department of Agriculture Washington, D. C., USA. |
| [32] | Okigbo R. N. and Osuinde M. I. (2003): Fungal leaf spot diseases of mango (Mangifera indica L.) in Southeastern Ni¬geria and biological control with Bacillus subtilis. Plant Protect. Sci., 39: 70–77. |
| [33] | Weir B. S., Johnston P. R. and Damm., U (2012). The Colletotrichum gloeosporioides species complex. Studies in Mycology. 73, 115-180. |
| [34] | Ikediugwu F. E. O., Emoghene A. O. and Ajiodo P. O. (1994). Biological control of the shoot disease of Amaranthus hybridus caused by Choanephora curcubitarum with Bacillus subtilis. J. Hort. Sci., 69:351-356. |
| [35] | Photita, W., Taylor, P. W. J., Ford, R., Hyde, K. D. and Lumyong, S. (2005). Morphological and molecular characterization of Colletotrichum species from herbaceous plants in Thailand. Fungal Diversity 18: 117-133. |
| [36] | Koomen I., Jeffries P. (1993). Effects of antagonistic microorganisms on the post-harvest development of Colletotrichum gloeosporioides on mango. Plant Pathology 42 (2): 230–237. |
| [37] | Santos-Villalobos S. de los, Guzmán-Ortiz D. A., Gómez-Lim M. A., Délano-Frier J. P., de-Folter S., Sánchez-García P., Peña-Cabriales J. J. (2013). Potential use of Trichoderma asperellum (Samuels, Liechfeldt et Nirenberg) T8a as a biological control agent against anthracnose in mango (Mangifera indica L.). Biological Control 64 (1): 37–44. |
| [38] | Bautista-Rosales P. U., Calderon-Santoyo M., Servín-Villegas R., Ochoa-Álvarez N. A., Vázquez-Juárez R., Ragazzo-Sánchez J. A. (2014). Biocontrol action mechanisms of Cryptococcus laurentii on Colletotrichum gloeosporioides on mango. Crop Protection 65 (1): 194–201. |
| [39] | Traquair J. A., Singh B., Sabaratnam S. (2013). Streptomyces biocontrol of thielaviopsis and Colletotrichum root rot in tomato transplants and Fusarium fruit rot in sweet bell pepper in greenhouses. Canadian Journal of Plant Pathology 35 (1):127–128. |
APA Style
Getachew Gashaw Dagnew. (2019). Morphological Characterization of Fungal Leaf Spot Diseases of Mango (Mangifera Indica L.) and in Vitro Evaluation of the Effect of Antagonists on Its Mycelial Growth. American Journal of Biomedical and Life Sciences, 6(6), 118-126. https://doi.org/10.11648/j.ajbls.20180606.12
ACS Style
Getachew Gashaw Dagnew. Morphological Characterization of Fungal Leaf Spot Diseases of Mango (Mangifera Indica L.) and in Vitro Evaluation of the Effect of Antagonists on Its Mycelial Growth. Am. J. Biomed. Life Sci. 2019, 6(6), 118-126. doi: 10.11648/j.ajbls.20180606.12
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Download@article{10.11648/j.ajbls.20180606.12,
author = {Getachew Gashaw Dagnew},
title = {Morphological Characterization of Fungal Leaf Spot Diseases of Mango (Mangifera Indica L.) and in Vitro Evaluation of the Effect of Antagonists on Its Mycelial Growth},
journal = {American Journal of Biomedical and Life Sciences},
volume = {6},
number = {6},
pages = {118-126},
doi = {10.11648/j.ajbls.20180606.12},
url = {https://doi.org/10.11648/j.ajbls.20180606.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20180606.12},
abstract = {The incidence of fungal leaf spot diseases on mango (Mangifera indica) in Oda Bultum University Mango farm and application of fungicide and a biological control measure was investigated. In addition microbial biocontrol and fungicide control against C. gloeosporioides developed for mangoes has been less reported in Ethiopia. Common mango disease control mechanisms in Ethiopia are utilization of pesticides. However, pesticides are overused and misused. As a result there is an urgent need to reduce the use of synthetic chemicals. Biological control offers an alternative to the use of pesticides. Invitro evaluation and testing of T. harzianum showed maximum mycelial growth inhibition (74.4% and 73.5%) on C. gloeosporioides isolates 1 and 3 respectively. The minimum percent of mycelial growth inhibition (71.5%) of T. harzianum was observed on isolate 1. Pseudomonas fluorescens showed maximum mycelial growth inhibition on isolate 2 (74.1%), followed by isolate 1 (70.4%). Least percent of mycelial growth inhibition by P. fluorescens was observed on isolate 3 (69%). B. subtilis showed maximum mycelial growth inhibition on isolate 3 (56.1%), followed by isolate 1 (51.5%). Least percent of mycelial growth inhibition by B. subtilis was observed on isolate 2 (48.6%). In vitro evaluation of fungicides against the three isolated test pathogen revealed that at 1000 PPM, all fungicides showed highest percentage inhibition (72.8% to 90.14%). The highest percentage inhibition was observed at the concentration of1000 PPM on isolates 2 (90.14%) and 3 (88.13%) and the lowest percentage inhibition was observed at the concentration of 200 PPM on isolates 1 (71.46%) and 3 (77.18%). The highest percentage mycelial growth inhibition by Sancozeb 80% WP on the isolates was 90.14% at a concentration of 1000PPM, whereas the lowest percentage mycelia growth inhibition of 83.06% was recorded at 200PPM. The highest percentage mycelial growth inhibition by Ridomil on the isolates was (83.16%) at 1000 PPM and the lowest percentage mycelial growth inhibition was recorded on isolate 1 (72.83%) at 200 PPM. Among the two fungicides Sancozeb was the most effective fungicide to inhibit the growth of the isolates with 87.98-90.14% inhibition.},
year = {2019}
}
TY - JOUR T1 - Morphological Characterization of Fungal Leaf Spot Diseases of Mango (Mangifera Indica L.) and in Vitro Evaluation of the Effect of Antagonists on Its Mycelial Growth AU - Getachew Gashaw Dagnew Y1 - 2019/01/15 PY - 2019 N1 - https://doi.org/10.11648/j.ajbls.20180606.12 DO - 10.11648/j.ajbls.20180606.12 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 118 EP - 126 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20180606.12 AB - The incidence of fungal leaf spot diseases on mango (Mangifera indica) in Oda Bultum University Mango farm and application of fungicide and a biological control measure was investigated. In addition microbial biocontrol and fungicide control against C. gloeosporioides developed for mangoes has been less reported in Ethiopia. Common mango disease control mechanisms in Ethiopia are utilization of pesticides. However, pesticides are overused and misused. As a result there is an urgent need to reduce the use of synthetic chemicals. Biological control offers an alternative to the use of pesticides. Invitro evaluation and testing of T. harzianum showed maximum mycelial growth inhibition (74.4% and 73.5%) on C. gloeosporioides isolates 1 and 3 respectively. The minimum percent of mycelial growth inhibition (71.5%) of T. harzianum was observed on isolate 1. Pseudomonas fluorescens showed maximum mycelial growth inhibition on isolate 2 (74.1%), followed by isolate 1 (70.4%). Least percent of mycelial growth inhibition by P. fluorescens was observed on isolate 3 (69%). B. subtilis showed maximum mycelial growth inhibition on isolate 3 (56.1%), followed by isolate 1 (51.5%). Least percent of mycelial growth inhibition by B. subtilis was observed on isolate 2 (48.6%). In vitro evaluation of fungicides against the three isolated test pathogen revealed that at 1000 PPM, all fungicides showed highest percentage inhibition (72.8% to 90.14%). The highest percentage inhibition was observed at the concentration of1000 PPM on isolates 2 (90.14%) and 3 (88.13%) and the lowest percentage inhibition was observed at the concentration of 200 PPM on isolates 1 (71.46%) and 3 (77.18%). The highest percentage mycelial growth inhibition by Sancozeb 80% WP on the isolates was 90.14% at a concentration of 1000PPM, whereas the lowest percentage mycelia growth inhibition of 83.06% was recorded at 200PPM. The highest percentage mycelial growth inhibition by Ridomil on the isolates was (83.16%) at 1000 PPM and the lowest percentage mycelial growth inhibition was recorded on isolate 1 (72.83%) at 200 PPM. Among the two fungicides Sancozeb was the most effective fungicide to inhibit the growth of the isolates with 87.98-90.14% inhibition. VL - 6 IS - 6 ER -
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