The Erythrocyte Binding Antigen-175 (EBA-175) on Plasmodium falciparum (P.falciparum) merozoites mediates sialic acid dependent binding to glycophorin a on host erythrocytes and, therefore, plays a crucial role in cell invasion. Dimorphic allele segments have been found in its encoding gene with in FCR-3 strains (F-segment) and CAMP strains (C-segment). This study was designed to determine the distribution of EBA-175 alleles of P.falciparum in the South-East of Iran. Nested Polymerase Chain Reaction method used with specific primers, which improves the two fragments of the EBA-175 gene. Ninety-four microscopically positive blood samples were collected from the infected P.falciparum malaria patients from four different districts. The 88 out of 94 confirmed P.falciparum samples were successfully scored for EBA-175. The allelic genotyping exhibited CAMP strains (714 bp) and FCR-3 strains (795 bp) in 31(32.97%) and 49(52.12%) cases, respectively and 8 cases (8.51%) showed mixed allelic CAMP/FCR-3 infections. The two fragments of dimorphic EBA-175 gene were observed and the FCR-3 allele was more prevalent in South-East of Iran. This distributional pattern should be considered in designing to control P. falciparum malaria in the region.
| Published in | American Journal of BioScience (Volume 2, Issue 2) |
| DOI | 10.11648/j.ajbio.20140202.16 |
| Page(s) | 56-59 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Plasmodium falciparum, Erythrocyte Binding Antigen-175, South-East of Iran
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APA Style
Ebrahimzadeh Adel, Jamshidi Ali. (2014). Genotyping of Erythrocyte Binding Antigen 175 KD (eba-175) Alleles of Plasmodium Falciparum-Malaria Using Nested-PCR in South-East of Iran. American Journal of BioScience, 2(2), 56-59. https://doi.org/10.11648/j.ajbio.20140202.16
ACS Style
Ebrahimzadeh Adel; Jamshidi Ali. Genotyping of Erythrocyte Binding Antigen 175 KD (eba-175) Alleles of Plasmodium Falciparum-Malaria Using Nested-PCR in South-East of Iran. Am. J. BioScience 2014, 2(2), 56-59. doi: 10.11648/j.ajbio.20140202.16
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Download@article{10.11648/j.ajbio.20140202.16,
author = {Ebrahimzadeh Adel and Jamshidi Ali},
title = {Genotyping of Erythrocyte Binding Antigen 175 KD (eba-175) Alleles of Plasmodium Falciparum-Malaria Using Nested-PCR in South-East of Iran},
journal = {American Journal of BioScience},
volume = {2},
number = {2},
pages = {56-59},
doi = {10.11648/j.ajbio.20140202.16},
url = {https://doi.org/10.11648/j.ajbio.20140202.16},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140202.16},
abstract = {The Erythrocyte Binding Antigen-175 (EBA-175) on Plasmodium falciparum (P.falciparum) merozoites mediates sialic acid dependent binding to glycophorin a on host erythrocytes and, therefore, plays a crucial role in cell invasion. Dimorphic allele segments have been found in its encoding gene with in FCR-3 strains (F-segment) and CAMP strains (C-segment). This study was designed to determine the distribution of EBA-175 alleles of P.falciparum in the South-East of Iran. Nested Polymerase Chain Reaction method used with specific primers, which improves the two fragments of the EBA-175 gene. Ninety-four microscopically positive blood samples were collected from the infected P.falciparum malaria patients from four different districts. The 88 out of 94 confirmed P.falciparum samples were successfully scored for EBA-175. The allelic genotyping exhibited CAMP strains (714 bp) and FCR-3 strains (795 bp) in 31(32.97%) and 49(52.12%) cases, respectively and 8 cases (8.51%) showed mixed allelic CAMP/FCR-3 infections. The two fragments of dimorphic EBA-175 gene were observed and the FCR-3 allele was more prevalent in South-East of Iran. This distributional pattern should be considered in designing to control P. falciparum malaria in the region.},
year = {2014}
}
TY - JOUR T1 - Genotyping of Erythrocyte Binding Antigen 175 KD (eba-175) Alleles of Plasmodium Falciparum-Malaria Using Nested-PCR in South-East of Iran AU - Ebrahimzadeh Adel AU - Jamshidi Ali Y1 - 2014/04/10 PY - 2014 N1 - https://doi.org/10.11648/j.ajbio.20140202.16 DO - 10.11648/j.ajbio.20140202.16 T2 - American Journal of BioScience JF - American Journal of BioScience JO - American Journal of BioScience SP - 56 EP - 59 PB - Science Publishing Group SN - 2330-0167 UR - https://doi.org/10.11648/j.ajbio.20140202.16 AB - The Erythrocyte Binding Antigen-175 (EBA-175) on Plasmodium falciparum (P.falciparum) merozoites mediates sialic acid dependent binding to glycophorin a on host erythrocytes and, therefore, plays a crucial role in cell invasion. Dimorphic allele segments have been found in its encoding gene with in FCR-3 strains (F-segment) and CAMP strains (C-segment). This study was designed to determine the distribution of EBA-175 alleles of P.falciparum in the South-East of Iran. Nested Polymerase Chain Reaction method used with specific primers, which improves the two fragments of the EBA-175 gene. Ninety-four microscopically positive blood samples were collected from the infected P.falciparum malaria patients from four different districts. The 88 out of 94 confirmed P.falciparum samples were successfully scored for EBA-175. The allelic genotyping exhibited CAMP strains (714 bp) and FCR-3 strains (795 bp) in 31(32.97%) and 49(52.12%) cases, respectively and 8 cases (8.51%) showed mixed allelic CAMP/FCR-3 infections. The two fragments of dimorphic EBA-175 gene were observed and the FCR-3 allele was more prevalent in South-East of Iran. This distributional pattern should be considered in designing to control P. falciparum malaria in the region. VL - 2 IS - 2 ER -
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