Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients

Liu Guihua; Huang Xin; Zhang Weiyu; Wang Yanqiu

doi:doi:10.11648/j.sjph.20200803.13

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Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients

Liu Guihua, Huang Xin, Zhang Weiyu, Wang Yanqiu

Published in Science Journal of Public Health (Volume 8, Issue 3)

Received: 6 May 2020 Accepted: 22 May 2020 Published: 4 June 2020

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Abstract

Objective: To detect the drug resistance of Salmonella isolated from the faeces samples of patients with food-borne diseases, and to know the drug resistance status and development trend of food-borne pathogens in our province. Methods: Enrichment, color medium separation, API20E biochemistry and Thai serum Identification. The minimal inhibitory concentration (MIC) of Salmonella to 15 kinds of antibiotics were quantitatively determined by micro broth dilution method. Results: 123 food-borne Salmonella strains were resistant to ampicillin (AMP), NALIDIXIC acid (NAL), tetracycline (TET), trimethoprim/sulfamethoxazole (SXT), ampicillin / sulbactam (AMS), CEFAZOLIN (CFZ), chloramphenicol (CHL), CEFOTAXIME (CTX), ciprofloxacin (CIP), azithromycin (AZM), gentamicin (GEN), cefoxitin (CFX), ceftazidime (CAZ). The drug resistance rates were 87.80%, 84.6%, 69.1%, 50.4%, 39.0%, 34.1%, 26.0%, 26.0%, 26.0%, 24.3%, 19.5%, 2.4% and 0.8% respectively. IMP and ERY were both sensitive. Among them, 90 strains were resistant to more than 3 kinds of antibiotics, and the multidrug resistance rate was 73.2%. MIC results ranged from 1μg / mL to > 64 μg / mL. The total strains were divided into 15 serotypings, including Salmonella Enteritidis (58.5%), Salmonella Typhimurium (16.3%), and Salmonella Stanley (4.9%). The most common distribution of the 57 resistant strains were AMP-TET-NAL-and AMP-NAL-11 strains, followed by NAL-10 strains, AMP-AMS-TET-NAL-7 strains, and multi-resistant strains ranged from 3 to 11 antibiotics. Conclusion: The serotypings of Salmonella isolated from faeces samples of patients from food borne disease surveillance sites in hospitals in Jilin province were mainly S. Enteritis and S. Enterica, and the strains were resistant to multiple antibiotics. The surveillance of drug resistance of Salmonella in food-borne diseases should be strengthened to ensure food safety and human health.

Published in	Science Journal of Public Health (Volume 8, Issue 3)
DOI	10.11648/j.sjph.20200803.13
Page(s)	77-82
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Foodborne Diseases, Broth Dilution, MIC, Salmonella, Results Analysis

References

[1]	Zhang Ling, Liu Guihua. Quantitative detection and Risk Assessment of Salmonella in chicken carcasses [J]. Chinese Journal of microecology, 2015, v 27 (8): 907-909.
[2]	Du Xiongwei, Jiang Jie, Li Yan, etc. Detection and analysis of drug resistance of Salmonella Suis [J]. Journal of Dalian Nationalities University, 2017, v 19 (1): 33-35.
[3]	Liu Guihua, Guo Changhong, Zhou Fengyan, etc. Analysis of enterococcus contamination and detection results in animal food chain in Jilin Province [J]. China health engineering, 2019, v 18 (6): 815-818.
[4]	Liu Guihua, Huang Xin, Zhang Weiyu, Gong Yunwei. Detection and analysis of drug resistance of Salmonella in stool of patients with food-borne diseases [J]. Chinese journal of public health engingeering 2018, v. 17 (01): 25-28.
[5]	Li Yueting, Gong Yunwei, Liu Guihua. Analysis of drug resistance of food-borne Salmonella and laxative ESCHERICHIA coli [J]. Chinese Journal of Health Inspection, 2016, v. 26 (22): 3335-3337.
[6]	Ministry of Health of the People's Republic of China. GB 4789.4-2016 National Standard for Food Safety Food Microbiology, Salmonella [S]. Beijing: China Standard Publishing, 2017.
[7]	Chaudun, Liu Guihua, Qu Li. Analysis of detection results of Salmonella by MIC drug sensitive kit of broth dilution method [J]. Chinese journal of public health engingeering, 2014, v. 13 (4): 314-319.
[8]	Li Shunji, Liu Guihua. Detection and analysis of drug resistance of Listeria monocytogene in foods with monocyte proliferation [J]. Chinese Journal of Health Inspection, 2015, v. 25 (20): 3772 -3774.
[9]	Li Yueting, Gong Yunwei, Liu Guihua. Analysis of drug resistance of Staphylococcus aureus in Jilin Province in 2014 [J]. Chinese Journal of Health Inspection, 2015, v. 25 (21): 3586-3587.
[10]	Zhao Wei, Liu Guihua, Wang Yanqiu, etc. Investigation on contamination and drug resistance of Liszt in food [J]. Chinese Journal of Health Inspection, 2012, v. 22 (6): 1394-1395.
[11]	Li Shunji, Wang Shudong, Cao Tiehong, Yin Dexiu, Chen Shengchen, Liu Guihua. Drug sensitivity test and result analysis of 41 strains of diarrhea-causing ESCHERICHIA coli [J]. Chinese journal of public health engingeering, 2016, v. 15 (2): 127-129.
[12]	Ren Huijing, Liu Yun. Current status of research on drug resistance of foodborne pathogens [J]. Henan Agriculture, 2013, No. 324 (16): 57+59.
[13]	Yang Yang, Fu Ping, Guo Yunchang, etc. Trend of Listeria monocytogene resistance in food-borne mononucleosis in China in 2005 [J]. Health Research, 2008, v. 37 (2): 183-186.
[14]	Cui Huixia, Li Wenjie, Zhang Xiuli, etc. SEROTYPING and drug resistance analysis of food-borne Liszt in Henan Province in 2012 [J]. Chinese Journal of Health Inspection, 2014, v. 24 (12): 1800-1803.
[15]	Xu Yunmin, Du Yan, Dan Bin, etc.. Surveillance of drug resistance of Salmonella Spp. in CHINET from 2005 to 2014 [J]. Chinese Journal of Infection and chemotherapy, 2016, v. 16 (3): 294 -301.
[16]	Wang Fang. Research progress of multi-drug Resistance Gene Island 1 of Salmonella [J]. Chinese Journal of Antibiotics, 2010, v. 35 (06): 414-420.
[17]	Zhang Rui, Zhao Xueqin, Shang Xiaochun, etc.. Composition and drug resistance of pathogenic bacteria of infantile diarrhea in Hangzhou [J]. Chinese Journal of Health Inspection, 2015, v. 25 (06): 901-903+905.

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APA Style

Liu Guihua, Huang Xin, Zhang Weiyu, Wang Yanqiu. (2020). Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients. Science Journal of Public Health, 8(3), 77-82. https://doi.org/10.11648/j.sjph.20200803.13

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ACS Style

Liu Guihua; Huang Xin; Zhang Weiyu; Wang Yanqiu. Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients. Sci. J. Public Health 2020, 8(3), 77-82. doi: 10.11648/j.sjph.20200803.13

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AMA Style

Liu Guihua, Huang Xin, Zhang Weiyu, Wang Yanqiu. Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients. Sci J Public Health. 2020;8(3):77-82. doi: 10.11648/j.sjph.20200803.13

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@article{10.11648/j.sjph.20200803.13,
  author = {Liu Guihua and Huang Xin and Zhang Weiyu and Wang Yanqiu},
  title = {Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients},
  journal = {Science Journal of Public Health},
  volume = {8},
  number = {3},
  pages = {77-82},
  doi = {10.11648/j.sjph.20200803.13},
  url = {https://doi.org/10.11648/j.sjph.20200803.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20200803.13},
  abstract = {Objective: To detect the drug resistance of Salmonella isolated from the faeces samples of patients with food-borne diseases, and to know the drug resistance status and development trend of food-borne pathogens in our province. Methods: Enrichment, color medium separation, API20E biochemistry and Thai serum Identification. The minimal inhibitory concentration (MIC) of Salmonella to 15 kinds of antibiotics were quantitatively determined by micro broth dilution method. Results: 123 food-borne Salmonella strains were resistant to ampicillin (AMP), NALIDIXIC acid (NAL), tetracycline (TET), trimethoprim/sulfamethoxazole (SXT), ampicillin / sulbactam (AMS), CEFAZOLIN (CFZ), chloramphenicol (CHL), CEFOTAXIME (CTX), ciprofloxacin (CIP), azithromycin (AZM), gentamicin (GEN), cefoxitin (CFX), ceftazidime (CAZ). The drug resistance rates were 87.80%, 84.6%, 69.1%, 50.4%, 39.0%, 34.1%, 26.0%, 26.0%, 26.0%, 24.3%, 19.5%, 2.4% and 0.8% respectively. IMP and ERY were both sensitive. Among them, 90 strains were resistant to more than 3 kinds of antibiotics, and the multidrug resistance rate was 73.2%. MIC results ranged from 1μg / mL to > 64 μg / mL. The total strains were divided into 15 serotypings, including Salmonella Enteritidis (58.5%), Salmonella Typhimurium (16.3%), and Salmonella Stanley (4.9%). The most common distribution of the 57 resistant strains were AMP-TET-NAL-and AMP-NAL-11 strains, followed by NAL-10 strains, AMP-AMS-TET-NAL-7 strains, and multi-resistant strains ranged from 3 to 11 antibiotics. Conclusion: The serotypings of Salmonella isolated from faeces samples of patients from food borne disease surveillance sites in hospitals in Jilin province were mainly S. Enteritis and S. Enterica, and the strains were resistant to multiple antibiotics. The surveillance of drug resistance of Salmonella in food-borne diseases should be strengthened to ensure food safety and human health.},
 year = {2020}
}

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TY - JOUR
T1 - Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients
AU - Liu Guihua
AU - Huang Xin
AU - Zhang Weiyu
AU - Wang Yanqiu
Y1 - 2020/06/04
PY - 2020
N1 - https://doi.org/10.11648/j.sjph.20200803.13
DO - 10.11648/j.sjph.20200803.13
T2 - Science Journal of Public Health
JF - Science Journal of Public Health
JO - Science Journal of Public Health
SP - 77
EP - 82
PB - Science Publishing Group
SN - 2328-7950
UR - https://doi.org/10.11648/j.sjph.20200803.13
AB - Objective: To detect the drug resistance of Salmonella isolated from the faeces samples of patients with food-borne diseases, and to know the drug resistance status and development trend of food-borne pathogens in our province. Methods: Enrichment, color medium separation, API20E biochemistry and Thai serum Identification. The minimal inhibitory concentration (MIC) of Salmonella to 15 kinds of antibiotics were quantitatively determined by micro broth dilution method. Results: 123 food-borne Salmonella strains were resistant to ampicillin (AMP), NALIDIXIC acid (NAL), tetracycline (TET), trimethoprim/sulfamethoxazole (SXT), ampicillin / sulbactam (AMS), CEFAZOLIN (CFZ), chloramphenicol (CHL), CEFOTAXIME (CTX), ciprofloxacin (CIP), azithromycin (AZM), gentamicin (GEN), cefoxitin (CFX), ceftazidime (CAZ). The drug resistance rates were 87.80%, 84.6%, 69.1%, 50.4%, 39.0%, 34.1%, 26.0%, 26.0%, 26.0%, 24.3%, 19.5%, 2.4% and 0.8% respectively. IMP and ERY were both sensitive. Among them, 90 strains were resistant to more than 3 kinds of antibiotics, and the multidrug resistance rate was 73.2%. MIC results ranged from 1μg / mL to > 64 μg / mL. The total strains were divided into 15 serotypings, including Salmonella Enteritidis (58.5%), Salmonella Typhimurium (16.3%), and Salmonella Stanley (4.9%). The most common distribution of the 57 resistant strains were AMP-TET-NAL-and AMP-NAL-11 strains, followed by NAL-10 strains, AMP-AMS-TET-NAL-7 strains, and multi-resistant strains ranged from 3 to 11 antibiotics. Conclusion: The serotypings of Salmonella isolated from faeces samples of patients from food borne disease surveillance sites in hospitals in Jilin province were mainly S. Enteritis and S. Enterica, and the strains were resistant to multiple antibiotics. The surveillance of drug resistance of Salmonella in food-borne diseases should be strengthened to ensure food safety and human health.
VL - 8
IS - 3
ER -

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Author Information

Liu Guihua

Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
Huang Xin

Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
Zhang Weiyu

Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
Wang Yanqiu

Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China

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Plain Text BibTeX RIS

APA Style

Liu Guihua, Huang Xin, Zhang Weiyu, Wang Yanqiu. (2020). Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients. Science Journal of Public Health, 8(3), 77-82. https://doi.org/10.11648/j.sjph.20200803.13

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ACS Style

Liu Guihua; Huang Xin; Zhang Weiyu; Wang Yanqiu. Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients. Sci. J. Public Health 2020, 8(3), 77-82. doi: 10.11648/j.sjph.20200803.13

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AMA Style

Liu Guihua, Huang Xin, Zhang Weiyu, Wang Yanqiu. Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients. Sci J Public Health. 2020;8(3):77-82. doi: 10.11648/j.sjph.20200803.13

Copy | Download

@article{10.11648/j.sjph.20200803.13,
  author = {Liu Guihua and Huang Xin and Zhang Weiyu and Wang Yanqiu},
  title = {Detection and Analysis of Antimicrobial Resistance of Salmonella in Faeces of Foodborne Disease Patients},
  journal = {Science Journal of Public Health},
  volume = {8},
  number = {3},
  pages = {77-82},
  doi = {10.11648/j.sjph.20200803.13},
  url = {https://doi.org/10.11648/j.sjph.20200803.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20200803.13},
  abstract = {Objective: To detect the drug resistance of Salmonella isolated from the faeces samples of patients with food-borne diseases, and to know the drug resistance status and development trend of food-borne pathogens in our province. Methods: Enrichment, color medium separation, API20E biochemistry and Thai serum Identification. The minimal inhibitory concentration (MIC) of Salmonella to 15 kinds of antibiotics were quantitatively determined by micro broth dilution method. Results: 123 food-borne Salmonella strains were resistant to ampicillin (AMP), NALIDIXIC acid (NAL), tetracycline (TET), trimethoprim/sulfamethoxazole (SXT), ampicillin / sulbactam (AMS), CEFAZOLIN (CFZ), chloramphenicol (CHL), CEFOTAXIME (CTX), ciprofloxacin (CIP), azithromycin (AZM), gentamicin (GEN), cefoxitin (CFX), ceftazidime (CAZ). The drug resistance rates were 87.80%, 84.6%, 69.1%, 50.4%, 39.0%, 34.1%, 26.0%, 26.0%, 26.0%, 24.3%, 19.5%, 2.4% and 0.8% respectively. IMP and ERY were both sensitive. Among them, 90 strains were resistant to more than 3 kinds of antibiotics, and the multidrug resistance rate was 73.2%. MIC results ranged from 1μg / mL to > 64 μg / mL. The total strains were divided into 15 serotypings, including Salmonella Enteritidis (58.5%), Salmonella Typhimurium (16.3%), and Salmonella Stanley (4.9%). The most common distribution of the 57 resistant strains were AMP-TET-NAL-and AMP-NAL-11 strains, followed by NAL-10 strains, AMP-AMS-TET-NAL-7 strains, and multi-resistant strains ranged from 3 to 11 antibiotics. Conclusion: The serotypings of Salmonella isolated from faeces samples of patients from food borne disease surveillance sites in hospitals in Jilin province were mainly S. Enteritis and S. Enterica, and the strains were resistant to multiple antibiotics. The surveillance of drug resistance of Salmonella in food-borne diseases should be strengthened to ensure food safety and human health.},
 year = {2020}
}

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