High Frequency of Multiple Shoots Induction in Paederia Foetida (L.)- A Rare Medicinal Plant

M. Thirupathi; D. Srinivas; K. Jaganmohan Reddy

doi:doi:10.11648/j.plant.20130105.13

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High Frequency of Multiple Shoots Induction in Paederia Foetida (L.)- A Rare Medicinal Plant

M. Thirupathi, D. Srinivas, K. Jaganmohan Reddy

Published in Plant (Volume 1, Issue 5)

Received: 11 November 2013 Published: 30 December 2013

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Abstract

Plants have been the eternal source of food and medicine since antiquity in all traditions and cultures. The medicinal plants are considered as one of the most vital components of ecosystem and biodiversity. Tissue culture techniques are usually used for the propagation as well as conservation of medicinal plants. In vitro multiplication of shoot induction and other tissue culture studies is hither unknown in this species. In this study, experiments were designed to develop regeneration protocols for the propagation as well as conservation of a rare medicinal plant, Paederia foetida (L.) The high frequency of multiple shoots was recorded from direct organogenesis of nodal explant. MS medium supplemented with benzyl adenine (4.4µM), kinetin (4.6µM), and 3% sucrose promoted the maximum number of shoots as well as beneficial shoot length. The direct organogenesis in TDZ (2.2µM) containing medium gave maximum percentage of shoots in MS medium from nodal segments compared with that of the other growth regulators. These plants grew normally without showing any morphological variations from the mother plant.

Published in	Plant (Volume 1, Issue 5)
DOI	10.11648/j.plant.20130105.13
Page(s)	60-65
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Direct Organogenesis, Multiple Shoots, Paederia foetida, Rare Medicinal Plant, Regeneration

References

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APA Style

M. Thirupathi, D. Srinivas, K. Jaganmohan Reddy. (2013). High Frequency of Multiple Shoots Induction in Paederia Foetida (L.)- A Rare Medicinal Plant. Plant, 1(5), 60-65. https://doi.org/10.11648/j.plant.20130105.13

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ACS Style

M. Thirupathi; D. Srinivas; K. Jaganmohan Reddy. High Frequency of Multiple Shoots Induction in Paederia Foetida (L.)- A Rare Medicinal Plant. Plant. 2013, 1(5), 60-65. doi: 10.11648/j.plant.20130105.13

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AMA Style

M. Thirupathi, D. Srinivas, K. Jaganmohan Reddy. High Frequency of Multiple Shoots Induction in Paederia Foetida (L.)- A Rare Medicinal Plant. Plant. 2013;1(5):60-65. doi: 10.11648/j.plant.20130105.13

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@article{10.11648/j.plant.20130105.13,
  author = {M. Thirupathi and D. Srinivas and K. Jaganmohan Reddy},
  title = {High Frequency of Multiple Shoots Induction in Paederia Foetida (L.)- A Rare Medicinal Plant},
  journal = {Plant},
  volume = {1},
  number = {5},
  pages = {60-65},
  doi = {10.11648/j.plant.20130105.13},
  url = {https://doi.org/10.11648/j.plant.20130105.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20130105.13},
  abstract = {Plants have been the eternal source of food and medicine since antiquity in all traditions and cultures. The medicinal plants are considered as one of the most vital components of ecosystem and biodiversity. Tissue culture techniques are usually used for the propagation as well as conservation of medicinal plants. In vitro multiplication of shoot induction and other tissue culture studies is hither unknown in this species. In this study, experiments were designed to develop regeneration protocols for the propagation as well as conservation of a rare medicinal plant, Paederia foetida (L.) The high frequency of multiple shoots was recorded from direct organogenesis of nodal explant. MS medium supplemented with benzyl adenine (4.4µM), kinetin (4.6µM), and 3% sucrose promoted the maximum number of shoots as well as beneficial shoot length. The direct organogenesis in TDZ (2.2µM) containing medium gave maximum percentage of shoots in MS medium from nodal segments compared with that of the other growth regulators. These plants grew normally without showing any morphological variations from the mother plant.},
 year = {2013}
}

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TY - JOUR
T1 - High Frequency of Multiple Shoots Induction in Paederia Foetida (L.)- A Rare Medicinal Plant
AU - M. Thirupathi
AU - D. Srinivas
AU - K. Jaganmohan Reddy
Y1 - 2013/12/30
PY - 2013
N1 - https://doi.org/10.11648/j.plant.20130105.13
DO - 10.11648/j.plant.20130105.13
T2 - Plant
JF - Plant
JO - Plant
SP - 60
EP - 65
PB - Science Publishing Group
SN - 2331-0677
UR - https://doi.org/10.11648/j.plant.20130105.13
AB - Plants have been the eternal source of food and medicine since antiquity in all traditions and cultures. The medicinal plants are considered as one of the most vital components of ecosystem and biodiversity. Tissue culture techniques are usually used for the propagation as well as conservation of medicinal plants. In vitro multiplication of shoot induction and other tissue culture studies is hither unknown in this species. In this study, experiments were designed to develop regeneration protocols for the propagation as well as conservation of a rare medicinal plant, Paederia foetida (L.) The high frequency of multiple shoots was recorded from direct organogenesis of nodal explant. MS medium supplemented with benzyl adenine (4.4µM), kinetin (4.6µM), and 3% sucrose promoted the maximum number of shoots as well as beneficial shoot length. The direct organogenesis in TDZ (2.2µM) containing medium gave maximum percentage of shoots in MS medium from nodal segments compared with that of the other growth regulators. These plants grew normally without showing any morphological variations from the mother plant.
VL - 1
IS - 5
ER -

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Author Information

M. Thirupathi

Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal, Andhra Pradesh, India
D. Srinivas

Department of Botany, Telangana University, Nizamabad, Andhra Pradesh, India
K. Jaganmohan Reddy

Plant Tissue Culture Laboratory, Department of Botany, Kakatiya University, Warangal, Andhra Pradesh, India

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Plain Text BibTeX RIS

APA Style

M. Thirupathi, D. Srinivas, K. Jaganmohan Reddy. (2013). High Frequency of Multiple Shoots Induction in Paederia Foetida (L.)- A Rare Medicinal Plant. Plant, 1(5), 60-65. https://doi.org/10.11648/j.plant.20130105.13

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ACS Style

M. Thirupathi; D. Srinivas; K. Jaganmohan Reddy. High Frequency of Multiple Shoots Induction in Paederia Foetida (L.)- A Rare Medicinal Plant. Plant. 2013, 1(5), 60-65. doi: 10.11648/j.plant.20130105.13

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AMA Style

M. Thirupathi, D. Srinivas, K. Jaganmohan Reddy. High Frequency of Multiple Shoots Induction in Paederia Foetida (L.)- A Rare Medicinal Plant. Plant. 2013;1(5):60-65. doi: 10.11648/j.plant.20130105.13

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@article{10.11648/j.plant.20130105.13,
  author = {M. Thirupathi and D. Srinivas and K. Jaganmohan Reddy},
  title = {High Frequency of Multiple Shoots Induction in Paederia Foetida (L.)- A Rare Medicinal Plant},
  journal = {Plant},
  volume = {1},
  number = {5},
  pages = {60-65},
  doi = {10.11648/j.plant.20130105.13},
  url = {https://doi.org/10.11648/j.plant.20130105.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20130105.13},
  abstract = {Plants have been the eternal source of food and medicine since antiquity in all traditions and cultures. The medicinal plants are considered as one of the most vital components of ecosystem and biodiversity. Tissue culture techniques are usually used for the propagation as well as conservation of medicinal plants. In vitro multiplication of shoot induction and other tissue culture studies is hither unknown in this species. In this study, experiments were designed to develop regeneration protocols for the propagation as well as conservation of a rare medicinal plant, Paederia foetida (L.) The high frequency of multiple shoots was recorded from direct organogenesis of nodal explant. MS medium supplemented with benzyl adenine (4.4µM), kinetin (4.6µM), and 3% sucrose promoted the maximum number of shoots as well as beneficial shoot length. The direct organogenesis in TDZ (2.2µM) containing medium gave maximum percentage of shoots in MS medium from nodal segments compared with that of the other growth regulators. These plants grew normally without showing any morphological variations from the mother plant.},
 year = {2013}
}

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