The abundant bioresources found in nature, including plants are used in the green synthesis of Silver nanoparticles. It offers an improvement over chemical and physical processes since it is less expensive and more environmentally friendly. This study aimed to synthesize silver nanoparticle using green method, characterization of silver nanoparticle using analytical techniques and to evaluate the antibacterial activity of synthesized silver nanoparticle. Psidium guajava L. leaf was collected, authenticated and extracted by water. A 1mM solution of silver nitrate was prepared. Afterwards, separate quantities of 1, 2, 3, 4, and 5mL of the plant extract were added to 10mL of silver nitrate to facilitate the synthesis of silver nanoparticles (AgNPs). The synthesized AgNps were characterized using UV/VIS Spectrometry, SEM analysis and FTIR analysis, also the antibacterial activity of AgNP at different concentrations against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa was evaluated using well diffusion method. The synthesized Silver nanoparticle were detected by UV/ Vis spectrophotometer with absorbance peak at 459 nm. SEM analysis showed that silver nanoparticles were crystalline aggregate in nature and the size of AgNP was estimated between 1 - 200 nm. The reduction of silver nanoparticles and presence of functional groups of plant extract was confirmed by FTIR analysis of silver nanoparticles. AgNPs synthesized from P. guajava leaf revealed antimicrobial activity against all selected bacterial species. The use of nanotechnology in therapy development is an incredibly exciting and rapidly advancing field, the potential benefits of these emerging technologies make them a highly promising avenue for future medical research and innovation.
| Published in | American Journal of Nano Research and Applications (Volume 11, Issue 2) |
| DOI | 10.11648/j.nano.20231102.11 |
| Page(s) | 19-25 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2023. Published by Science Publishing Group |
Nanotechnology, Green Synthesis, Nanoparticles, Guava, Antibacterial Activity
| [1] | Silva GA. Nanotechnology applications and approaches for neuroregeneration and drug delivery to the central nervous system. Annals of the New York Academy of Sciences. 2010 Jun; 1199 (1): 221-30. |
| [2] | Mitiku AA, Yilma B. A review on green synthesis and antibacterial activity of silver nanoparticles. Int. J. Pharm. Sci. Rev. Res. 2018; 46: 52-7. |
| [3] | Bala M, Arya V. Biological synthesis of silver nanoparticles from aqueous extract of endophytic fungus Aspergillus Fumigatus and its antibacterial action. International Journal of Nanomaterials and Biostructures. 2013; 3 (2): 37-41. |
| [4] | Coccia M, Finardi U, Margon D. Current trends in nanotechnology research across worldwide geo-economic players. The Journal of Technology Transfer. 2012 Oct; 37: 777-87. |
| [5] | Rai, M., Ingle, A. P., Gaikwad, S., Gupta, I., Gade, A., &Silvério da Silva, S. Nanotechnology based anti-infectives to fight microbial intrusions. Journal of Applied Microbiology. 2016; 120 (3), 527–542. |
| [6] | Huh, A. J., & Kwon, Y. J. “Nanoantibiotics”: a new paradigm for treating infectious diseases using nanomaterials in the antibiotics resistant era. Journal of Controlled Release: Official Journal of the Controlled Release Society. 2011; 156 (2), 128–145. |
| [7] | Gutiérrez RMP, Mitchell S, Solis RV. Psidium guajava: a review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol. 2008; 117 (1): 1–27. |
| [8] | Begum S, Hassan SI, Siddiqui BS, Two new triterpenoids from the leaves from Psidium guajava, Phytochemistry. 2002; 61, 399-403. |
| [9] | Ougandhi PR, Reddeppaand M, Harini SS, Shobha Rani T, Gangadhara R, Green synthesis and characterization of silver nanoparticles by using Psidium guajava leaf extract, Journal of Drug Delivery and Therapeutics. 2018; 8 (5-s): 301-305. |
| [10] | Elakkiya GT, Balaji GL, Padhy H, Lakshmipathy R. Synthesis of silver nanoplates using regenerated watermelon rind and their application. Materials Today: Proceedings. 2022 Jan 1; 55: 240-5. |
| [11] | Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. Journal of pharmaceutical analysis. 2016, 1; 6 (2): 71-9. |
| [12] | Ranjan, P., Das, M. P., Kumar, M. S., Anbarasi, P., Sindhu, S., Sagadevan, E. and Arumugam, P. Green Synthesis and Characterization of Silver Nanoparticles from Nigella sativa and Its Application against UTI Causing Bacteria. Journal of Academia and Industrial Research. 2013; 2, 45-49. |
| [13] | Kumar TS, Rathimeena T, Thangapandian V, Shankar T. Silver nanoparticles synthesis of Menthaarvensis extracts and evaluation of antioxidant properties. BiosciBioeng. 2015; 1 (2): 22–8. |
| [14] | Prabu JH, Johnson I. Antibacterial activity of silver nanoparticles synthesized from plant leaf extract of Cycascircinalis, Ficusamplissima, Commelinabenghalensis and Lippianodiflora leaves. J Chem Pharm Res. 2015; 7 (9): 443–9. |
| [15] | Makabenta JM, Nabawy A, Li CH, Schmidt-Malan S, Patel R, Rotello VM. Nanomaterial-based therapeutics for antibiotic-resistant bacterial infections. Nature Reviews Microbiology. 2021 Jan; 19 (1): 23-36. |
| [16] | Kaviya S, Santhanalakshmi J, Viswanathan B, Muthumary J, Srinivasan K. Biosynthesis of silver nanoparticles using Citrus sinensis peel extract and its antibacterial activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2011 Aug 1; 79 (3): 594-8. |
| [17] | Kora AJ, Sashidhar RB. Biogenic silver nanoparticles synthesized with rhamnogalacturonan gum: Antibacterial activity, cytotoxicity and its mode of action. Arabian Journal of Chemistry. 2018 Mar 1; 11 (3): 313-23. |
| [18] | Nisar P, Ali N, Rahman L, Ali M, Shinwari ZK. Antimicrobial activities of biologically synthesized metal nanoparticles: an insight into the mechanism of action. JBIC Journal of Biological Inorganic Chemistry. 2019 Oct; 24: 929-41. |
| [19] | Ali M, Yahaya A, Zage A, Yusuf Z. In-vitro antibacterial activity and phytochemical screening of Psidium guajava on some enteric bacterial isolates of public health importance. Journal of Advances in Medical and Pharmaceutical Sciences. 2017 Jan 10; 12 (3): 1-7. |
| [20] | Biswas B, Rogers K, McLaughlin F, Daniels D, Yadav A. Antimicrobial activities of leaf extracts of guava (Psidium guajava L.) on two gram-negative and gram-positive bacteria. International journal of microbiology. 2013 Oct 20; 2013. |
| [21] | Patra JK, Das G, Fraceto LF, Campos EV, Rodriguez-Torres MD, Acosta-Torres LS, Diaz-Torres LA, Grillo R, Swamy MK, Sharma S, Habtemariam S. Nano based drug delivery systems: recent developments and future prospects. Journal of nanobiotechnology. 2018 Dec; 16 (1): 1-33. |
| [22] | Mishra AC, Chaubey R, Srivastava SP. Implementation of nanotechnology in healthcare: immense challenges and opportunities. Biological Sciences. 2022 Jul 29; 2 (3): 251-61. |
| [23] | Anjum S, Ishaque S, Fatima H, Farooq W, Hano C, Abbasi BH, Anjum I. Emerging applications of nanotechnology in healthcare systems: Grand challenges and perspectives. Pharmaceuticals. 2021 Jul 21; 14 (8): 707. |
| [24] | Sahoo BM, Kumar BVVR, Patra CN, Panda JR, Mohanta BC, Palei NN. Nanotechnology: A novel approach for drug development in health care system. Currnanomater [Internet]. 2020; 5 (1): 12–25. |
APA Style
Tasabeeh Omer Abdulazeez, Ayat Ahmed Alrasheid, Ahmed Saeed Kabbashi, Saad Mohammed Hussein Ayoub. (2023). Green Synthesis of Silver Nanoparticles from Psidium guajava Leaf Extract: Characterization and Evaluation of Antibacterial Activity Against Common Pathogens. American Journal of Nano Research and Applications, 11(2), 19-25. https://doi.org/10.11648/j.nano.20231102.11
ACS Style
Tasabeeh Omer Abdulazeez; Ayat Ahmed Alrasheid; Ahmed Saeed Kabbashi; Saad Mohammed Hussein Ayoub. Green Synthesis of Silver Nanoparticles from Psidium guajava Leaf Extract: Characterization and Evaluation of Antibacterial Activity Against Common Pathogens. Am. J. Nano Res. Appl. 2023, 11(2), 19-25. doi: 10.11648/j.nano.20231102.11
AMA Style
Tasabeeh Omer Abdulazeez, Ayat Ahmed Alrasheid, Ahmed Saeed Kabbashi, Saad Mohammed Hussein Ayoub. Green Synthesis of Silver Nanoparticles from Psidium guajava Leaf Extract: Characterization and Evaluation of Antibacterial Activity Against Common Pathogens. Am J Nano Res Appl. 2023;11(2):19-25. doi: 10.11648/j.nano.20231102.11
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Download@article{10.11648/j.nano.20231102.11,
author = {Tasabeeh Omer Abdulazeez and Ayat Ahmed Alrasheid and Ahmed Saeed Kabbashi and Saad Mohammed Hussein Ayoub},
title = {Green Synthesis of Silver Nanoparticles from Psidium guajava Leaf Extract: Characterization and Evaluation of Antibacterial Activity Against Common Pathogens},
journal = {American Journal of Nano Research and Applications},
volume = {11},
number = {2},
pages = {19-25},
doi = {10.11648/j.nano.20231102.11},
url = {https://doi.org/10.11648/j.nano.20231102.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.nano.20231102.11},
abstract = {The abundant bioresources found in nature, including plants are used in the green synthesis of Silver nanoparticles. It offers an improvement over chemical and physical processes since it is less expensive and more environmentally friendly. This study aimed to synthesize silver nanoparticle using green method, characterization of silver nanoparticle using analytical techniques and to evaluate the antibacterial activity of synthesized silver nanoparticle. Psidium guajava L. leaf was collected, authenticated and extracted by water. A 1mM solution of silver nitrate was prepared. Afterwards, separate quantities of 1, 2, 3, 4, and 5mL of the plant extract were added to 10mL of silver nitrate to facilitate the synthesis of silver nanoparticles (AgNPs). The synthesized AgNps were characterized using UV/VIS Spectrometry, SEM analysis and FTIR analysis, also the antibacterial activity of AgNP at different concentrations against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa was evaluated using well diffusion method. The synthesized Silver nanoparticle were detected by UV/ Vis spectrophotometer with absorbance peak at 459 nm. SEM analysis showed that silver nanoparticles were crystalline aggregate in nature and the size of AgNP was estimated between 1 - 200 nm. The reduction of silver nanoparticles and presence of functional groups of plant extract was confirmed by FTIR analysis of silver nanoparticles. AgNPs synthesized from P. guajava leaf revealed antimicrobial activity against all selected bacterial species. The use of nanotechnology in therapy development is an incredibly exciting and rapidly advancing field, the potential benefits of these emerging technologies make them a highly promising avenue for future medical research and innovation.},
year = {2023}
}
TY - JOUR T1 - Green Synthesis of Silver Nanoparticles from Psidium guajava Leaf Extract: Characterization and Evaluation of Antibacterial Activity Against Common Pathogens AU - Tasabeeh Omer Abdulazeez AU - Ayat Ahmed Alrasheid AU - Ahmed Saeed Kabbashi AU - Saad Mohammed Hussein Ayoub Y1 - 2023/08/22 PY - 2023 N1 - https://doi.org/10.11648/j.nano.20231102.11 DO - 10.11648/j.nano.20231102.11 T2 - American Journal of Nano Research and Applications JF - American Journal of Nano Research and Applications JO - American Journal of Nano Research and Applications SP - 19 EP - 25 PB - Science Publishing Group SN - 2575-3738 UR - https://doi.org/10.11648/j.nano.20231102.11 AB - The abundant bioresources found in nature, including plants are used in the green synthesis of Silver nanoparticles. It offers an improvement over chemical and physical processes since it is less expensive and more environmentally friendly. This study aimed to synthesize silver nanoparticle using green method, characterization of silver nanoparticle using analytical techniques and to evaluate the antibacterial activity of synthesized silver nanoparticle. Psidium guajava L. leaf was collected, authenticated and extracted by water. A 1mM solution of silver nitrate was prepared. Afterwards, separate quantities of 1, 2, 3, 4, and 5mL of the plant extract were added to 10mL of silver nitrate to facilitate the synthesis of silver nanoparticles (AgNPs). The synthesized AgNps were characterized using UV/VIS Spectrometry, SEM analysis and FTIR analysis, also the antibacterial activity of AgNP at different concentrations against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa was evaluated using well diffusion method. The synthesized Silver nanoparticle were detected by UV/ Vis spectrophotometer with absorbance peak at 459 nm. SEM analysis showed that silver nanoparticles were crystalline aggregate in nature and the size of AgNP was estimated between 1 - 200 nm. The reduction of silver nanoparticles and presence of functional groups of plant extract was confirmed by FTIR analysis of silver nanoparticles. AgNPs synthesized from P. guajava leaf revealed antimicrobial activity against all selected bacterial species. The use of nanotechnology in therapy development is an incredibly exciting and rapidly advancing field, the potential benefits of these emerging technologies make them a highly promising avenue for future medical research and innovation. VL - 11 IS - 2 ER -
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