Legume consumption has been consistently linked with lower risk of cardiovascular disease (CVD) and Coronary heart disease (CHD), as a result from their unique phytochemicals. Studies investigating phytochemical profiles and antioxidant activity of legumes in Botswana are limited. Five legume varieties were studied. All the legumes showed a significant amount of total phenolic acids and flavonoids ranging from 64.83 to 828.69 mg of gallic acid equiv/100 g of sample, DW and from 85.36 to 410.99 ± 21.24 mg of catechin equiv/100 g of sample, DW respectively. Their antioxidant activity ranged from 50.7 to 114.6 mg vitamin C /100g of DW. In this study, there was a positive correlation between TPCs and PSC value of the samples (R2=0.9940, P<0.01). The higher TPCs resulted in higher antioxidant activity, an indication that phenolics were the major contributors to antioxidant activities. Chlorogenic, caffeic, p-coumaric, and ferulic acid were detected in all Cowpea varieties (Cowpea-Thamagana Speckle, Cowpea-Inia, and Cowpea-Red). The results from the study emphasize the importance of these legumes as a source of phenolic acids and antioxidants which could contribute to their health promoting properties and prevention of some diseases.
Published in | Journal of Food and Nutrition Sciences (Volume 8, Issue 4) |
DOI | 10.11648/j.jfns.20200804.15 |
Page(s) | 103-107 |
Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2020. Published by Science Publishing Group |
Legumes, Phytochemicals, Phenolics, Flavonoids, Antioxidant Activity, HPLC
[1] | Bouchenak, M., Lamri-Senhadji, “Nutritional quality of legumes and their role in cardiometabolic risk prevention: a review”, Journal of Medicinal Foods, 16 (3): 185-198. 2013. |
[2] | Odhav, B., Beekrum. S., Akula. U., Baijnath, H, “Preliminary assessment of nutritional value of traditional leafy vegetables in KwaZulu-Natal, South Africa”, Journal of Food Composition and Analysis, 20, 430- 435. 2007. |
[3] | Butt, M. S., & Batool, R, “Nutritional and functional Properties of Some Promising Legumes Protein Isolates”, Pakistan Journal of Nutrition, 9 (4): 373-379. 2010. |
[4] | Marathe, S., Rajalakshmi, S. N., Sahayog, N. J., Sharma, A, “Comparative study on antioxidant activity of different varieties of commonly consumed legumes in India” Food and Chemical Toxicology, 49: 2005-2012. 2011. |
[5] | Amarteifio, J. O., Moholo, D, “The chemical composition of four legumes consumed in Botswana”. Journal of Food Composition and Analysis, 11: 329-332. 1998. |
[6] | Graham, O. H., Vance, C. P, “Legumes: Importance and constraints to greater use:”. Plant Physiology, 131 (3): 872-877. 2003. |
[7] | Campos-Vega, R., Guadalupe, L., Oomah, B, “Minor Components of Pulses and Their Potential Impact on Human Health”. Food Research International, 43 (2): 461–482. 2010. |
[8] | Liu, R. H, “Potential synergy of phytochemicals in cancer prevention: Mechanism of action”, Journal of Nutrition, 134 (12), 3479S-3485S. 2004. |
[9] | Amarowicz, R., Pegg, R. B, “Legumes as source of natural antioxidants”, European Journal of Lipid Science and Technology, 110: 865-878. 2008. |
[10] | Liu, R. H, “Health benefits of fruits and vegetables are from addictive and synergistic combinations of phytochemicals”, American Journal of Clinical Nutrition, 78 (3), 517S-520S. 2003. |
[11] | Flight, I., Clifton, P, “Cereal grains and legumes in the prevention of coronary heart disease and stroke: a review” European Journal of Clinical Nutrition, 60 (10): 1145-59. 2006. |
[12] | Cardador-Martinez, A., Loarca-pina, G., Oomah, B. D, “Antioxidant activity in common beans (Phaseolus vulgaris L.)”, J Agricultural Food Chemistry, 50: 6975-6980. 2006. |
[13] | Messina, M. J, “Legumes and soybeans: Overview of their nutritional profiles and health effects” American Journal Clinical Nutritional, 70 (3): 439S-450S. 1999. |
[14] | Pusztai, A., Grant, G., Buchan, W. C. Bardocz, A. F. F. U., Ewen, B, “Lipid accumulation in obese Zucker rats is reduced by inclusion of raw kidney bean (Phaseolus vulgaris) in the diet”, British Journal of Nutrition, 79: 213–221. 1998. |
[15] | Singleton VL; Orthofer R, Lamuela-Raventos RM, “Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent method”. Enzyme, 299: 152–178. 1999. |
[16] | Adom, K. K., Liu, R. H, “Rapid peroxyl radical scavenging capacity (PSC) assay for assessing both hydrophilic and lipophilic antioxidants” Journal of Agriculture and Food Chemistry, 53 (17): 6572-80. 2005. |
[17] | He, X. Liu, D., Liu, R. H, “Sodium borohydride/chloranil-based assay for quantifying total flavonoids”, Journal of Agriculture and Food Chemistry, 56, 9337-9344. 2008. |
[18] | Mesquita, F. R.; Correa, A. D.; Abreu, C. M. P.; Lima, R. A. Z.; Abreu, A. F. B, “Linhagens de feijão (Phaseolus vulgaris L.) composição química e digestibilidade protéica”. Ciencia e Agrotecnologia, 31 (4): 1114-1121. 2007. |
[19] | Cavalcante, R. B. M., Araújo, M. A. D. M., Rocha, M. M., Silva, K. J. D. Moreira-Araújo, R. S. R, “Effect of thermal processing on total polyphenol content in the grain of cowpea cultivars”. Revista Ciência Agronômica, 48 (5), 806-810. |
[20] | Felice, D. L., Sun, J., & Liu, R. H. (2009). A modified methylene blue assay for accurate cell counting. Journal of Functional Foods, 1, 109−118. |
[21] | Luo, Y. W., & Xie, W. H. (2012). Effects of vegetables on iron and zinc availability in cereals and legumes. International Food Research Journal, 19, 455-459. |
[22] | Yao, Y., Cheng X., Wang, L., Wang, S., & Ren, G. (2011). Biological Potential of Sixteen Legumes in China. International Journal of Molecular Sciences, 12, 7048-7058. |
[23] | Ren, S., Liu, Z., & Wang. P. (2012). Proximate composition and flavonoids content and in vitro antioxidant activity of 10 varieties of legume seeds grown in China. Journal of Medical Plants, 6 (2), 301-308. |
[24] | Gujral, S., Sharma, P., Gupta, N. & Wani, A. A. (2013). Antioxidant properties of Legumes and their morphological fractions as affected by cooking. Food Science and Biotechnology, 22 (1), 187-194. |
[25] | Adom, K. K.; Sorrel, M. E.; Liu, R. H. (2005). Phytochemicals and antioxidant activity of milled fractions of different wheat varieties. Journal of Agricultural and Food Chemistry, 53 (6), 2297-306. |
[26] | Awika, J. M., Rooney, L. W., Wu, X., Prior, R. L., & Zevallos, L. C. (2003). Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products. Journal of Agricultural and Food Chemistry, 51, 6657–6662. |
[27] | Wojdyło, A., Oszmian, J., Czemerys, R., Antioxidant activity of phenolic acids in 32 selected herbs. Food Chemistry, 105, 940-949. Jan 2001 |
[28] | Mathew, J, Abraham TE, & Zakaria, ZA. (2015). Reactivity of phenolic compounds towards free radicals under in vitro conditions. Journal of Food Science and Technology, 52 (9), 5790-5798 |
[29] | Li, H., Cao, D., Yi, J., Cao, J., & Jiang, W. (2012). Identification of the flavonoids in mung bean (Phaseolus radiatus L.) soup and their antioxidant activities. Food Chemistry, 135 (4), 2942– 2946. |
[30] | Guajardo-Flores, D., García-Patiño, M., Serna-Guerrero, D., Gutiérrez-Uribe, J. A., & Serna-Saldívar, S. O. (2012). Characterization and quantification of saponins and flavonoids in sprouts, seed coats and cotyledons of germinated black beans. Food Chemistry, 134, 1312–1319. |
[31] | Owino, J., Mukashyaka, P., Ndayisaba, H., Habimana, V., Ongol, M. P., Thavarajah, D., & Pushparajah, T. (2014). Phenolic Compound Profiles of Two Common Beans Consumed by Rwandans. American Journal of Plant Science, 5, 2943-2947. |
[32] | Luthria, A., Singh, K., & D’souza, M. (2014). In Vitro antioxidant activity of black gram, cowpea, desi chickpea and yellow mustard as affected by sprouting. Journal of Global Biosciences, 3 (1), 385-389. |
APA Style
Sarah Tshepho Pona Matenge. (2020). Phytochemical Profiles and Antioxidant Activity of Legumes Consumed in Botswana. Journal of Food and Nutrition Sciences, 8(4), 103-107. https://doi.org/10.11648/j.jfns.20200804.15
ACS Style
Sarah Tshepho Pona Matenge. Phytochemical Profiles and Antioxidant Activity of Legumes Consumed in Botswana. J. Food Nutr. Sci. 2020, 8(4), 103-107. doi: 10.11648/j.jfns.20200804.15
AMA Style
Sarah Tshepho Pona Matenge. Phytochemical Profiles and Antioxidant Activity of Legumes Consumed in Botswana. J Food Nutr Sci. 2020;8(4):103-107. doi: 10.11648/j.jfns.20200804.15
@article{10.11648/j.jfns.20200804.15, author = {Sarah Tshepho Pona Matenge}, title = {Phytochemical Profiles and Antioxidant Activity of Legumes Consumed in Botswana}, journal = {Journal of Food and Nutrition Sciences}, volume = {8}, number = {4}, pages = {103-107}, doi = {10.11648/j.jfns.20200804.15}, url = {https://doi.org/10.11648/j.jfns.20200804.15}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20200804.15}, abstract = {Legume consumption has been consistently linked with lower risk of cardiovascular disease (CVD) and Coronary heart disease (CHD), as a result from their unique phytochemicals. Studies investigating phytochemical profiles and antioxidant activity of legumes in Botswana are limited. Five legume varieties were studied. All the legumes showed a significant amount of total phenolic acids and flavonoids ranging from 64.83 to 828.69 mg of gallic acid equiv/100 g of sample, DW and from 85.36 to 410.99 ± 21.24 mg of catechin equiv/100 g of sample, DW respectively. Their antioxidant activity ranged from 50.7 to 114.6 mg vitamin C /100g of DW. In this study, there was a positive correlation between TPCs and PSC value of the samples (R2=0.9940, Pp-coumaric, and ferulic acid were detected in all Cowpea varieties (Cowpea-Thamagana Speckle, Cowpea-Inia, and Cowpea-Red). The results from the study emphasize the importance of these legumes as a source of phenolic acids and antioxidants which could contribute to their health promoting properties and prevention of some diseases.}, year = {2020} }
TY - JOUR T1 - Phytochemical Profiles and Antioxidant Activity of Legumes Consumed in Botswana AU - Sarah Tshepho Pona Matenge Y1 - 2020/07/30 PY - 2020 N1 - https://doi.org/10.11648/j.jfns.20200804.15 DO - 10.11648/j.jfns.20200804.15 T2 - Journal of Food and Nutrition Sciences JF - Journal of Food and Nutrition Sciences JO - Journal of Food and Nutrition Sciences SP - 103 EP - 107 PB - Science Publishing Group SN - 2330-7293 UR - https://doi.org/10.11648/j.jfns.20200804.15 AB - Legume consumption has been consistently linked with lower risk of cardiovascular disease (CVD) and Coronary heart disease (CHD), as a result from their unique phytochemicals. Studies investigating phytochemical profiles and antioxidant activity of legumes in Botswana are limited. Five legume varieties were studied. All the legumes showed a significant amount of total phenolic acids and flavonoids ranging from 64.83 to 828.69 mg of gallic acid equiv/100 g of sample, DW and from 85.36 to 410.99 ± 21.24 mg of catechin equiv/100 g of sample, DW respectively. Their antioxidant activity ranged from 50.7 to 114.6 mg vitamin C /100g of DW. In this study, there was a positive correlation between TPCs and PSC value of the samples (R2=0.9940, Pp-coumaric, and ferulic acid were detected in all Cowpea varieties (Cowpea-Thamagana Speckle, Cowpea-Inia, and Cowpea-Red). The results from the study emphasize the importance of these legumes as a source of phenolic acids and antioxidants which could contribute to their health promoting properties and prevention of some diseases. VL - 8 IS - 4 ER -