<i>In Vitro antisickling</i> and Free Radical Scavenging Activities of <i>Kigelia africana</i> (LAM) Stem Bark

Tatiana Kangah Mireille Kple; Joel Akakpo-Akue; Koffi Adou Mathieu Kra; N’Guessan Bra Yvette Fofie; Koffi Julien Golly; Ibrahime Sanogo; Antoinette Claire Chiaye Yapo-Crezoit

doi:doi:10.11648/j.jdmp.20210701.11

In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark

Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Koffi Adou Mathieu Kra, N’Guessan Bra Yvette Fofie, Koffi Julien Golly, Ibrahime Sanogo, Antoinette Claire Chiaye Yapo-Crezoit

Published in Journal of Diseases and Medicinal Plants (Volume 7, Issue 1)

Received: 12 December 2020 Accepted: 30 December 2020 Published: 30 January 2021

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Abstract

Kigelia africana dried bark has been recommended in the management of sickle cell anemia by traditional practitioners. The aim of this study was to evaluate in vitro antioxidant and sickling inhibitory activities of Kigelia africana. Quantitative estimation of phenolic compounds was performed using colorimetric method in hydro-ethanolic extract (70%) and decocted. The sickling inhibitory activity was determinate according to Emmel method and the evaluation of antioxidant properties was carried out using the method of scavenging the free radical DPPH. The obtained results indicated that Kigelia africana species contains flavonoids (8.61±1.08 mg QE and 9.78±1.14 mg QE/g of dry weight) and total phenols (9.48±0.19b mg GAE/g and 11.11±0.22 mg GAE /g of dry weight) in decocted and hydroethanolic extract respectively. The IC₅₀ values of the ethanolic extract and the decocted were 0.320±0.01 and 0.468±0.04 mg/mL respectively. The in vitro sickle-formation inhibition test indicated the value of 89% and 82.36% for the ethanolic extract and the decocted at 10mg/mL respectively. Those values were higher than 80% which was the phenylalanine value. Both extracts showed antioxidant and sickling inhibitory activities. Overall, there could have a correlation between these activities and phenolic compound content in this studied plant extracts. These results would justify the use of this plant in rural environment.

Published in	Journal of Diseases and Medicinal Plants (Volume 7, Issue 1)
DOI	10.11648/j.jdmp.20210701.11
Page(s)	1-5
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Kigelia africana, Phenolic Compounds, Antisickling Activity, Antioxidant Activity, Cote d’Ivoire

References

[1]	Dro B, Soro D, Koné MW, Bakayoko A. and Kamanzi K. Evaluation of the abundance of medicinal plants used in traditional medicine in northern Cote d'Ivoire. Journal of Animal and Plant Sciences 2013, 17 (3): 2631-2646.
[2]	Gnagne AS, Camara D, Fofie N'GBY, Bene K and Zirihi GN. Ethnobotanical study of medicinal plants used in the treatment of diabetes in the Department of Zouenoula (Cote d'Ivoire). Journal of Applied Biosciences 2017, 113.
[3]	Akakpo-Akue J, Kplé T. K. M.., Coulibaly Kiyinlma, AHON Gnamien Marce, Fofie N’GBY, Yapo -Crezoit. ACC, Zirihi GN and Kra AKM. Ethnobotanical study of medicinal plants used against sickle cell anaemia in the eastern part of the Cote d’Ivoire. Journal of Animal & Plant Sciences 2020 45(1).
[4]	Mates JM and Sanchez-Jimenez FM. Role of reactive oxygen species in apoptosis: implications for cancer therapy. Int J Biochem Cell Biol 2000, 32: 157-170.
[5]	Nur E, Biemond BJ, Otten HM, Brandjes DP and Schnog JJ. Oxidative stress in sickle cell desease; pathophysiology and potential implications for desease management. American Journal Of Hematology 2011, 86 pp 484-489.
[6]	Hierso R. Implication of oxidative stress in the pathophysiology of sickle cell disease vaso-occlusive attacks, anti-band 3 antibody levels and red blood cell oxidation. Université des Antilles et de la Guyane, Faculté de Sciences exactes et naturelles, Pointe-à-Pitre, France 2015, 185 p.
[7]	Akintunde JK, Akintunde DG, Irondi EA, Babaita K, Babaita R and Sunday O. Antioxidants from stem bark of Kigelia africana inhibits free radicals and membrane lipid damage in rat testes in vitro. Oxidants and Antioxidants in Medical Science 2016.
[8]	Atawodi S, Ene-Ojo and Olufunsho DO. Pharmacological and Therapeutic Activities of Kigelia africana (Lam.) Benth. Annual Research & Review in Biology, 2015, 5(1): 1-17.
[9]	Zirihi G. Kra AKM and Guede-Guina, F. Evaluation of the antifungal activity of Microglossa pyrifolia (Lamarck) O. Kantze (Asteracee) PYMI on the in vitro growth of Candida albicans. Revue de Médecine et pharmacie Afrique 2003, 17:11-18.
[10]	Konkon NG, Adjoungoua AL, Manda P, Simaga D, N’Guessan KE. and Koné B. D. Toxicological and phytochemical screening study of MitragynaInermis (willd.) O ktze (Rubiaceae), anti-diabetic plant.. J. Med. Plant Res 2008, 2(10):279-284.
[11]	Singleton VL, Orthofer R and Lamuela- Raventos, RM. Analysis of total phenols and other oxydant substrates and antioxydants by means of Folin-ciocalteu reagent. Methods Enzymol 1999, 299: 152-178.
[12]	Meda A, Lamien CE, Romito M, Millogo J, and Nacoulma OG. Determination of total phenolic, flavonoid and proline contents in Burkina Faso honeys as well as well as their radical scavenging activity. Food. Chem 2005, 91:571-577.
[13]	Parejo I. Codina C, Petraski C. and Kefalas P. Evaluation of scavening activity assessed by co (II)/EDTA-induced luminal chemilunescence and DDPH (2,2-diphenyl-1-pycril hydrazyl) free radical assay. Journal of. Pharmacology and. Toxicology Method 2000, 44: 507-512.
[14]	Emmel VE. A red cell study of the érythrocytes in case of severe anemia with elongated and sickle and shape dred blood corpuscule. Archives of Internal Medicine (1933), 7: 769-789.
[15]	Mpiana PT, Misakabu FM, Yuma PM, Tshibangu DST, Ngbolua KN, Mwanyishay CL, Misengabu NM, Gbolo ZB and Kayembe JS. Antisickling Activity and Physico-chemical Stability of Anthocyanin Extracts from Ipomoea Batatas Leaves. JLM 2014, 2(1):25-31.
[16]	Essam Y. Abdul-Hafeez, Nazira S K and Olga NI. Antioxidant activity and total phenolic compound content of certain medicinal plants. International Journal of Biosciences 2014, 5(9), 213-222.
[17]	Abdulkadir N and Adedokun O. In Vitro Free Radical Scavenging Activity and Total Phenolic Content of Kigelia Africana (LAM). International Journal of Science and Research, 2014, 3 (1), 368-370.
[18]	Iram F, Tanveer H, Muhammad R, Muhammad A, Sabahat B and Sammia S. Evaluation of antioxidant activity of leaves and fruits extracts of five medicinal plants. Pak. J. Pharm. Sci 2017, 30 (5), 1625-1628.
[19]	Evenamede KS, Kpegba K, Simalou O, Boyode P, Agbonon A. and Gbeassor M. Comparative study of the antioxidant activities of ethanol extracts from leaves, bark and roots of Cassia sieberiana. Int. J. Biol. Chem. Sci 2017. 11(6): 2924-2935.
[20]	Sahgal G, Ramanathan S, Sasidharan S, Mordi MN, Ismail S and Mansor S M. In vitro antioxidant and xanthine oxidase inhibitory activities of methanolic Swietenia mahagoni seed extracts. Molecules 2009, 14(11), 4476-4485.
[21]	Kitadi DJM, Mazasa PP, Tshibangu DST, Memvanga PB, Ngbolua KN, Taba NK, Noki PV and Mpiana PT. Anti-sickling and antioxidant activities of anthocyanins extracts from Dissotis brazzae Cogn (Melastomataceae). Journal of advancement in medical and life sciences 2015, DOI: 10.13140/RG.2.1.2817.7363.
[22]	Kplé TKM, Akakpo-Akue J, Golly, JK, Fofie N’GBY, Y., Ahon MG, Kra MA, Sanogo I and Yapo-Crezoit ACC. Phytochemical Characterization of Three Plants and Their Antisickling Activity in the Management of Sickle Cell Disease. Journal of Biosciences and Medicines, 2020, 8, 100-112. https://doi.org/10.4236/jbm.2020.8601.
[23]	Jaldappagari S, Motohashi N, Gangeenahalli MP, Naismith JH. Bioactive mechanism of interaction between Anthocyanins and macromolecules like DNA and Proteins. In: N. Motohashi (Eds) Bioactive Heterocycles VI, Springer-Verlag Berlin Heidelberg 2008, pp. 49-65.
[24]	Cahyana Y. and Gordon MH. Interaction of anthocyanins with human serum albumin: Influence of pH and chemical structure on binding. Food Chemistry 2013, 141(3): 2278–2285.
[25]	Elion J. and Labie D. Molecular and cellular pathophysiological bases for the treatment of sickle cell disease. Haematology, 1996, 2: 499-510.
[26]	Weinman S. and Méhul P. Toute. Biochemistry. Paris Dunod, 2004, 466p.

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APA Style

Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Koffi Adou Mathieu Kra, N’Guessan Bra Yvette Fofie, Koffi Julien Golly, et al. (2021). In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark. Journal of Diseases and Medicinal Plants, 7(1), 1-5. https://doi.org/10.11648/j.jdmp.20210701.11

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Tatiana Kangah Mireille Kple; Joel Akakpo-Akue; Koffi Adou Mathieu Kra; N’Guessan Bra Yvette Fofie; Koffi Julien Golly, et al. In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark. J. Dis. Med. Plants 2021, 7(1), 1-5. doi: 10.11648/j.jdmp.20210701.11

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AMA Style

Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Koffi Adou Mathieu Kra, N’Guessan Bra Yvette Fofie, Koffi Julien Golly, et al. In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark. J Dis Med Plants. 2021;7(1):1-5. doi: 10.11648/j.jdmp.20210701.11

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@article{10.11648/j.jdmp.20210701.11,
  author = {Tatiana Kangah Mireille Kple and Joel Akakpo-Akue and Koffi Adou Mathieu Kra and N’Guessan Bra Yvette Fofie and Koffi Julien Golly and Ibrahime Sanogo and Antoinette Claire Chiaye Yapo-Crezoit},
  title = {In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {7},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.jdmp.20210701.11},
  url = {https://doi.org/10.11648/j.jdmp.20210701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20210701.11},
  abstract = {Kigelia africana dried bark has been recommended in the management of sickle cell anemia by traditional practitioners. The aim of this study was to evaluate in vitro antioxidant and sickling inhibitory activities of Kigelia africana. Quantitative estimation of phenolic compounds was performed using colorimetric method in hydro-ethanolic extract (70%) and decocted. The sickling inhibitory activity was determinate according to Emmel method and the evaluation of antioxidant properties was carried out using the method of scavenging the free radical DPPH. The obtained results indicated that Kigelia africana species contains flavonoids (8.61±1.08 mg QE and 9.78±1.14 mg QE/g of dry weight) and total phenols (9.48±0.19b mg GAE/g and 11.11±0.22 mg GAE /g of dry weight) in decocted and hydroethanolic extract respectively. The IC50 values of the ethanolic extract and the decocted were 0.320±0.01 and 0.468±0.04 mg/mL respectively. The in vitro sickle-formation inhibition test indicated the value of 89% and 82.36% for the ethanolic extract and the decocted at 10mg/mL respectively. Those values were higher than 80% which was the phenylalanine value. Both extracts showed antioxidant and sickling inhibitory activities. Overall, there could have a correlation between these activities and phenolic compound content in this studied plant extracts. These results would justify the use of this plant in rural environment.},
 year = {2021}
}

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TY - JOUR
T1 - In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark
AU - Tatiana Kangah Mireille Kple
AU - Joel Akakpo-Akue
AU - Koffi Adou Mathieu Kra
AU - N’Guessan Bra Yvette Fofie
AU - Koffi Julien Golly
AU - Ibrahime Sanogo
AU - Antoinette Claire Chiaye Yapo-Crezoit
Y1 - 2021/01/30
PY - 2021
N1 - https://doi.org/10.11648/j.jdmp.20210701.11
DO - 10.11648/j.jdmp.20210701.11
T2 - Journal of Diseases and Medicinal Plants
JF - Journal of Diseases and Medicinal Plants
JO - Journal of Diseases and Medicinal Plants
SP - 1
EP - 5
PB - Science Publishing Group
SN - 2469-8210
UR - https://doi.org/10.11648/j.jdmp.20210701.11
AB - Kigelia africana dried bark has been recommended in the management of sickle cell anemia by traditional practitioners. The aim of this study was to evaluate in vitro antioxidant and sickling inhibitory activities of Kigelia africana. Quantitative estimation of phenolic compounds was performed using colorimetric method in hydro-ethanolic extract (70%) and decocted. The sickling inhibitory activity was determinate according to Emmel method and the evaluation of antioxidant properties was carried out using the method of scavenging the free radical DPPH. The obtained results indicated that Kigelia africana species contains flavonoids (8.61±1.08 mg QE and 9.78±1.14 mg QE/g of dry weight) and total phenols (9.48±0.19b mg GAE/g and 11.11±0.22 mg GAE /g of dry weight) in decocted and hydroethanolic extract respectively. The IC50 values of the ethanolic extract and the decocted were 0.320±0.01 and 0.468±0.04 mg/mL respectively. The in vitro sickle-formation inhibition test indicated the value of 89% and 82.36% for the ethanolic extract and the decocted at 10mg/mL respectively. Those values were higher than 80% which was the phenylalanine value. Both extracts showed antioxidant and sickling inhibitory activities. Overall, there could have a correlation between these activities and phenolic compound content in this studied plant extracts. These results would justify the use of this plant in rural environment.
VL - 7
IS - 1
ER -

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Author Information

Tatiana Kangah Mireille Kple

Laboratory of Biology and Health, University Felix Houphouet-Boigny (UFHB), Abidjan, Cote d’Ivoire
Joel Akakpo-Akue

Laboratory of Biology and Health, University Felix Houphouet-Boigny (UFHB), Abidjan, Cote d’Ivoire
Koffi Adou Mathieu Kra

Laboratory of Biology and Health, University Felix Houphouet-Boigny (UFHB), Abidjan, Cote d’Ivoire
N’Guessan Bra Yvette Fofie

Pharmacognosy Laboratory, University Felix Houphouet-Boigny, Abidjan, Cote d’Ivoire
Koffi Julien Golly

Immunity Biology Center, Pasteur Institute of Cote d’Ivoire, Abidjan, Cote d’Ivoire
Ibrahime Sanogo

Department of Clinical Haematology, University Hospital of Yopougon, Abidjan, Cote d’Ivoire
Antoinette Claire Chiaye Yapo-Crezoit

Immunity Biology Center, Pasteur Institute of Cote d’Ivoire, Abidjan, Cote d’Ivoire

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APA Style

Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Koffi Adou Mathieu Kra, N’Guessan Bra Yvette Fofie, Koffi Julien Golly, et al. (2021). In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark. Journal of Diseases and Medicinal Plants, 7(1), 1-5. https://doi.org/10.11648/j.jdmp.20210701.11

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Tatiana Kangah Mireille Kple; Joel Akakpo-Akue; Koffi Adou Mathieu Kra; N’Guessan Bra Yvette Fofie; Koffi Julien Golly, et al. In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark. J. Dis. Med. Plants 2021, 7(1), 1-5. doi: 10.11648/j.jdmp.20210701.11

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AMA Style

Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Koffi Adou Mathieu Kra, N’Guessan Bra Yvette Fofie, Koffi Julien Golly, et al. In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark. J Dis Med Plants. 2021;7(1):1-5. doi: 10.11648/j.jdmp.20210701.11

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@article{10.11648/j.jdmp.20210701.11,
  author = {Tatiana Kangah Mireille Kple and Joel Akakpo-Akue and Koffi Adou Mathieu Kra and N’Guessan Bra Yvette Fofie and Koffi Julien Golly and Ibrahime Sanogo and Antoinette Claire Chiaye Yapo-Crezoit},
  title = {In Vitro antisickling and Free Radical Scavenging Activities of Kigelia africana (LAM) Stem Bark},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {7},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.jdmp.20210701.11},
  url = {https://doi.org/10.11648/j.jdmp.20210701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20210701.11},
  abstract = {Kigelia africana dried bark has been recommended in the management of sickle cell anemia by traditional practitioners. The aim of this study was to evaluate in vitro antioxidant and sickling inhibitory activities of Kigelia africana. Quantitative estimation of phenolic compounds was performed using colorimetric method in hydro-ethanolic extract (70%) and decocted. The sickling inhibitory activity was determinate according to Emmel method and the evaluation of antioxidant properties was carried out using the method of scavenging the free radical DPPH. The obtained results indicated that Kigelia africana species contains flavonoids (8.61±1.08 mg QE and 9.78±1.14 mg QE/g of dry weight) and total phenols (9.48±0.19b mg GAE/g and 11.11±0.22 mg GAE /g of dry weight) in decocted and hydroethanolic extract respectively. The IC50 values of the ethanolic extract and the decocted were 0.320±0.01 and 0.468±0.04 mg/mL respectively. The in vitro sickle-formation inhibition test indicated the value of 89% and 82.36% for the ethanolic extract and the decocted at 10mg/mL respectively. Those values were higher than 80% which was the phenylalanine value. Both extracts showed antioxidant and sickling inhibitory activities. Overall, there could have a correlation between these activities and phenolic compound content in this studied plant extracts. These results would justify the use of this plant in rural environment.},
 year = {2021}
}

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