Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells

Te-chun Hsia; Mei-chin Yin; Zhi-hong Wang

doi:doi:10.11648/j.cmr.20170605.11

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Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells

Te-chun Hsia, Mei-chin Yin, Zhi-hong Wang

Published in Clinical Medicine Research (Volume 6, Issue 5)

Received: 8 August 2017 Accepted: 7 September 2017 Published: 16 October 2017

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Abstract

Effects of carboxymethyllysine (CML), an advanced glycation end-product (AGE), at 1, 2, 4, 8 or 16 μmol/l upon invasion and migration of A549 cells, non-small cell lung cancer (NSCLC) cells, were investigated. Results showed that CML at used test doses did not affect A549 cell growth. However, CML at 4-16 μmol/l enhanced both invasion and migration, and stimulated the release of reactive oxygen species, interleukin-6 and tumor necrosis factor-alpha in A549 cells. CML at 2-16 μmol/l increased protein expression of AGE receptor, p47^phox, intercellular adhesion molecule-1, fibronectin, kappa-B (NF- κB) p65 and p-p38 in A549 cells. CML only at 4-16 μmol/l increased matrix metalloproteinase-2 expression in A549 cells. These findings indicated that CML might benefit NSCLC metastasis through promoting invasion and migration.

Published in	Clinical Medicine Research (Volume 6, Issue 5)
DOI	10.11648/j.cmr.20170605.11
Page(s)	149-156
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Carboxymethyllysine, Advanced Glycation End Products, NSCLC, A549 Cell, Migration, Invasion

References

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APA Style

Te-chun Hsia, Mei-chin Yin, Zhi-hong Wang. (2017). Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells. Clinical Medicine Research, 6(5), 149-156. https://doi.org/10.11648/j.cmr.20170605.11

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ACS Style

Te-chun Hsia; Mei-chin Yin; Zhi-hong Wang. Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells. Clin. Med. Res. 2017, 6(5), 149-156. doi: 10.11648/j.cmr.20170605.11

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AMA Style

Te-chun Hsia, Mei-chin Yin, Zhi-hong Wang. Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells. Clin Med Res. 2017;6(5):149-156. doi: 10.11648/j.cmr.20170605.11

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@article{10.11648/j.cmr.20170605.11,
  author = {Te-chun Hsia and Mei-chin Yin and Zhi-hong Wang},
  title = {Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells},
  journal = {Clinical Medicine Research},
  volume = {6},
  number = {5},
  pages = {149-156},
  doi = {10.11648/j.cmr.20170605.11},
  url = {https://doi.org/10.11648/j.cmr.20170605.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20170605.11},
  abstract = {Effects of carboxymethyllysine (CML), an advanced glycation end-product (AGE), at 1, 2, 4, 8 or 16 μmol/l upon invasion and migration of A549 cells, non-small cell lung cancer (NSCLC) cells, were investigated. Results showed that CML at used test doses did not affect A549 cell growth. However, CML at 4-16 μmol/l enhanced both invasion and migration, and stimulated the release of reactive oxygen species, interleukin-6 and tumor necrosis factor-alpha in A549 cells. CML at 2-16 μmol/l increased protein expression of AGE receptor, p47phox, intercellular adhesion molecule-1, fibronectin, kappa-B (NF- κB) p65 and p-p38 in A549 cells. CML only at 4-16 μmol/l increased matrix metalloproteinase-2 expression in A549 cells. These findings indicated that CML might benefit NSCLC metastasis through promoting invasion and migration.},
 year = {2017}
}

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TY  - JOUR
T1  - Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells
AU  - Te-chun Hsia
AU  - Mei-chin Yin
AU  - Zhi-hong Wang
Y1  - 2017/10/16
PY  - 2017
N1  - https://doi.org/10.11648/j.cmr.20170605.11
DO  - 10.11648/j.cmr.20170605.11
T2  - Clinical Medicine Research
JF  - Clinical Medicine Research
JO  - Clinical Medicine Research
SP  - 149
EP  - 156
PB  - Science Publishing Group
SN  - 2326-9057
UR  - https://doi.org/10.11648/j.cmr.20170605.11
AB  - Effects of carboxymethyllysine (CML), an advanced glycation end-product (AGE), at 1, 2, 4, 8 or 16 μmol/l upon invasion and migration of A549 cells, non-small cell lung cancer (NSCLC) cells, were investigated. Results showed that CML at used test doses did not affect A549 cell growth. However, CML at 4-16 μmol/l enhanced both invasion and migration, and stimulated the release of reactive oxygen species, interleukin-6 and tumor necrosis factor-alpha in A549 cells. CML at 2-16 μmol/l increased protein expression of AGE receptor, p47phox, intercellular adhesion molecule-1, fibronectin, kappa-B (NF- κB) p65 and p-p38 in A549 cells. CML only at 4-16 μmol/l increased matrix metalloproteinase-2 expression in A549 cells. These findings indicated that CML might benefit NSCLC metastasis through promoting invasion and migration.
VL  - 6
IS  - 5
ER  -

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Author Information

Te-chun Hsia

Department of Respiratory Therapy, China Medical University, Taichung City, Taiwan
Mei-chin Yin

Department of Food Nutrition and Health Biotechnology, Asia University, Taichung City, Taiwan
Zhi-hong Wang

Department of Food Nutrition and Health Biotechnology, Asia University, Taichung City, Taiwan

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Plain Text BibTeX RIS

APA Style

Te-chun Hsia, Mei-chin Yin, Zhi-hong Wang. (2017). Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells. Clinical Medicine Research, 6(5), 149-156. https://doi.org/10.11648/j.cmr.20170605.11

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ACS Style

Te-chun Hsia; Mei-chin Yin; Zhi-hong Wang. Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells. Clin. Med. Res. 2017, 6(5), 149-156. doi: 10.11648/j.cmr.20170605.11

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AMA Style

Te-chun Hsia, Mei-chin Yin, Zhi-hong Wang. Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells. Clin Med Res. 2017;6(5):149-156. doi: 10.11648/j.cmr.20170605.11

Copy | Download

@article{10.11648/j.cmr.20170605.11,
  author = {Te-chun Hsia and Mei-chin Yin and Zhi-hong Wang},
  title = {Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells},
  journal = {Clinical Medicine Research},
  volume = {6},
  number = {5},
  pages = {149-156},
  doi = {10.11648/j.cmr.20170605.11},
  url = {https://doi.org/10.11648/j.cmr.20170605.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20170605.11},
  abstract = {Effects of carboxymethyllysine (CML), an advanced glycation end-product (AGE), at 1, 2, 4, 8 or 16 μmol/l upon invasion and migration of A549 cells, non-small cell lung cancer (NSCLC) cells, were investigated. Results showed that CML at used test doses did not affect A549 cell growth. However, CML at 4-16 μmol/l enhanced both invasion and migration, and stimulated the release of reactive oxygen species, interleukin-6 and tumor necrosis factor-alpha in A549 cells. CML at 2-16 μmol/l increased protein expression of AGE receptor, p47phox, intercellular adhesion molecule-1, fibronectin, kappa-B (NF- κB) p65 and p-p38 in A549 cells. CML only at 4-16 μmol/l increased matrix metalloproteinase-2 expression in A549 cells. These findings indicated that CML might benefit NSCLC metastasis through promoting invasion and migration.},
 year = {2017}
}

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TY  - JOUR
T1  - Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells
AU  - Te-chun Hsia
AU  - Mei-chin Yin
AU  - Zhi-hong Wang
Y1  - 2017/10/16
PY  - 2017
N1  - https://doi.org/10.11648/j.cmr.20170605.11
DO  - 10.11648/j.cmr.20170605.11
T2  - Clinical Medicine Research
JF  - Clinical Medicine Research
JO  - Clinical Medicine Research
SP  - 149
EP  - 156
PB  - Science Publishing Group
SN  - 2326-9057
UR  - https://doi.org/10.11648/j.cmr.20170605.11
AB  - Effects of carboxymethyllysine (CML), an advanced glycation end-product (AGE), at 1, 2, 4, 8 or 16 μmol/l upon invasion and migration of A549 cells, non-small cell lung cancer (NSCLC) cells, were investigated. Results showed that CML at used test doses did not affect A549 cell growth. However, CML at 4-16 μmol/l enhanced both invasion and migration, and stimulated the release of reactive oxygen species, interleukin-6 and tumor necrosis factor-alpha in A549 cells. CML at 2-16 μmol/l increased protein expression of AGE receptor, p47phox, intercellular adhesion molecule-1, fibronectin, kappa-B (NF- κB) p65 and p-p38 in A549 cells. CML only at 4-16 μmol/l increased matrix metalloproteinase-2 expression in A549 cells. These findings indicated that CML might benefit NSCLC metastasis through promoting invasion and migration.
VL  - 6
IS  - 5
ER  -

Copy | Download