Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans

Vignatti Alicia M.; Cabrera Gabriela C.; Echaniz Santiago A.

doi:doi:10.11648/j.ajls.20140202.11

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Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans

Vignatti Alicia M., Cabrera Gabriela C., Echaniz Santiago A.

Published in American Journal of Life Sciences (Volume 2, Issue 2)

Received: 15 February 2014 Published: 10 March 2014

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Abstract

The neotropical halophilic cladocerans Daphnia menucoensis and Moina eugeniae are common in shallow lakes of semi-arid central and northern Patagonia in Argentina. In the present study, we determined the tolerance of neonates of both species to salinity and different concentrations of Cl- and SO42-. However, the responses differed according to the demineralized water or groundwater employed to dissolve the salts. On the other hand, survival was lower when using analytical grade reagents instead of natural salts. As bioassays should be developed to determine the chronic physiological responses of both species, the objectives of this study were to compare three culture media and test the hypothesis that natural salts dissolved in aquifer water is the optimal combination for breeding. Acute bioassays were conducted with neonates of both species. To compare the media, NaCl solutions (5, 10, 15, 20 and 25 g.L-1) were prepared using demineralized water, aquifer water and EPA medium. For the second objective, solutions of equal concentrations were prepared with salts obtained from the natural environment, previously sterilized, and dissolved in phreatic water. Survival was higher in the aquifer water, whose chemical composition was relatively more representative of the lakes inhabited by both species. It was almost complete at 20 and 15 g.L-1 for D. menucoensis and M. eugeniae, respectively. Lower survival of D. menucoensis was found in EPA medium, although it was developed for breeding other species of Daphnia. In the second series of bioassays, survival was almost complete until 20 g.L-1 (D. menucoensis) and 25 g.L-1 (M. eugeniae). The combination natural salts/aquifer water was adequate to assess the tolerance of both cladocerans. However, the former was more important, since the results were consistent with previous studies when these salts were dissolved in demineralized water. In addition, survival was much higher than when we used salts of analytical grade.

Published in	American Journal of Life Sciences (Volume 2, Issue 2)
DOI	10.11648/j.ajls.20140202.11
Page(s)	40-45
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Bioassays, Daphnia Menucoensis, Moina Eugeniae, Halophilic Cladocerans Culture, Salinity Tolerance

References

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Vignatti Alicia M., Cabrera Gabriela C., Echaniz Santiago A. (2014). Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans. American Journal of Life Sciences, 2(2), 40-45. https://doi.org/10.11648/j.ajls.20140202.11

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Vignatti Alicia M.; Cabrera Gabriela C.; Echaniz Santiago A. Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans. Am. J. Life Sci. 2014, 2(2), 40-45. doi: 10.11648/j.ajls.20140202.11

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Vignatti Alicia M., Cabrera Gabriela C., Echaniz Santiago A. Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans. Am J Life Sci. 2014;2(2):40-45. doi: 10.11648/j.ajls.20140202.11

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@article{10.11648/j.ajls.20140202.11,
  author = {Vignatti Alicia M. and Cabrera Gabriela C. and Echaniz Santiago A.},
  title = {Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {2},
  pages = {40-45},
  doi = {10.11648/j.ajls.20140202.11},
  url = {https://doi.org/10.11648/j.ajls.20140202.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140202.11},
  abstract = {The neotropical halophilic cladocerans Daphnia menucoensis and Moina eugeniae are common in shallow lakes of semi-arid central and northern Patagonia in Argentina. In the present study, we determined the tolerance of neonates of both species to salinity and different concentrations of Cl- and SO42-. However, the responses differed according to the demineralized water or groundwater employed to dissolve the salts. On the other hand, survival was lower when using analytical grade reagents instead of natural salts. As bioassays should be developed to determine the chronic physiological responses of both species, the objectives of this study were to compare three culture media and test the hypothesis that natural salts dissolved in aquifer water is the optimal combination for breeding. Acute bioassays were conducted with neonates of both species. To compare the media, NaCl solutions (5, 10, 15, 20 and 25 g.L-1) were prepared using demineralized water, aquifer water and EPA medium. For the second objective, solutions of equal concentrations were prepared with salts obtained from the natural environment, previously sterilized, and dissolved in phreatic water. Survival was higher in the aquifer water, whose chemical composition was relatively more representative of the lakes inhabited by both species. It was almost complete at 20 and 15 g.L-1 for D. menucoensis and M. eugeniae, respectively. Lower survival of D. menucoensis was found in EPA medium, although it was developed for breeding other species of Daphnia. In the second series of bioassays, survival was almost complete until 20 g.L-1 (D. menucoensis) and 25 g.L-1 (M. eugeniae). The combination natural salts/aquifer water was adequate to assess the tolerance of both cladocerans. However, the former was more important, since the results were consistent with previous studies when these salts were dissolved in demineralized water. In addition, survival was much higher than when we used salts of analytical grade.},
 year = {2014}
}

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TY - JOUR
T1 - Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans
AU - Vignatti Alicia M.
AU - Cabrera Gabriela C.
AU - Echaniz Santiago A.
Y1 - 2014/03/10
PY - 2014
N1 - https://doi.org/10.11648/j.ajls.20140202.11
DO - 10.11648/j.ajls.20140202.11
T2 - American Journal of Life Sciences
JF - American Journal of Life Sciences
JO - American Journal of Life Sciences
SP - 40
EP - 45
PB - Science Publishing Group
SN - 2328-5737
UR - https://doi.org/10.11648/j.ajls.20140202.11
AB - The neotropical halophilic cladocerans Daphnia menucoensis and Moina eugeniae are common in shallow lakes of semi-arid central and northern Patagonia in Argentina. In the present study, we determined the tolerance of neonates of both species to salinity and different concentrations of Cl- and SO42-. However, the responses differed according to the demineralized water or groundwater employed to dissolve the salts. On the other hand, survival was lower when using analytical grade reagents instead of natural salts. As bioassays should be developed to determine the chronic physiological responses of both species, the objectives of this study were to compare three culture media and test the hypothesis that natural salts dissolved in aquifer water is the optimal combination for breeding. Acute bioassays were conducted with neonates of both species. To compare the media, NaCl solutions (5, 10, 15, 20 and 25 g.L-1) were prepared using demineralized water, aquifer water and EPA medium. For the second objective, solutions of equal concentrations were prepared with salts obtained from the natural environment, previously sterilized, and dissolved in phreatic water. Survival was higher in the aquifer water, whose chemical composition was relatively more representative of the lakes inhabited by both species. It was almost complete at 20 and 15 g.L-1 for D. menucoensis and M. eugeniae, respectively. Lower survival of D. menucoensis was found in EPA medium, although it was developed for breeding other species of Daphnia. In the second series of bioassays, survival was almost complete until 20 g.L-1 (D. menucoensis) and 25 g.L-1 (M. eugeniae). The combination natural salts/aquifer water was adequate to assess the tolerance of both cladocerans. However, the former was more important, since the results were consistent with previous studies when these salts were dissolved in demineralized water. In addition, survival was much higher than when we used salts of analytical grade.
VL - 2
IS - 2
ER -

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Vignatti Alicia M.

Facultad de Ciencias Exactas y Naturales, Universidad Nacional de La Pampa, Santa Rosa, La Pampa, República Argentina
Cabrera Gabriela C.

Facultad de Ciencias Exactas y Naturales, Universidad Nacional de La Pampa, Santa Rosa, La Pampa, República Argentina
Echaniz Santiago A.

Facultad de Ciencias Exactas y Naturales, Universidad Nacional de La Pampa, Santa Rosa, La Pampa, República Argentina

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Vignatti Alicia M., Cabrera Gabriela C., Echaniz Santiago A. (2014). Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans. American Journal of Life Sciences, 2(2), 40-45. https://doi.org/10.11648/j.ajls.20140202.11

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Vignatti Alicia M.; Cabrera Gabriela C.; Echaniz Santiago A. Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans. Am. J. Life Sci. 2014, 2(2), 40-45. doi: 10.11648/j.ajls.20140202.11

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Vignatti Alicia M., Cabrera Gabriela C., Echaniz Santiago A. Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans. Am J Life Sci. 2014;2(2):40-45. doi: 10.11648/j.ajls.20140202.11

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@article{10.11648/j.ajls.20140202.11,
  author = {Vignatti Alicia M. and Cabrera Gabriela C. and Echaniz Santiago A.},
  title = {Determination of the Optimal Culture Medium and Salts for Bioassays with Neotropical Halophilic Cladocerans},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {2},
  pages = {40-45},
  doi = {10.11648/j.ajls.20140202.11},
  url = {https://doi.org/10.11648/j.ajls.20140202.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140202.11},
  abstract = {The neotropical halophilic cladocerans Daphnia menucoensis and Moina eugeniae are common in shallow lakes of semi-arid central and northern Patagonia in Argentina. In the present study, we determined the tolerance of neonates of both species to salinity and different concentrations of Cl- and SO42-. However, the responses differed according to the demineralized water or groundwater employed to dissolve the salts. On the other hand, survival was lower when using analytical grade reagents instead of natural salts. As bioassays should be developed to determine the chronic physiological responses of both species, the objectives of this study were to compare three culture media and test the hypothesis that natural salts dissolved in aquifer water is the optimal combination for breeding. Acute bioassays were conducted with neonates of both species. To compare the media, NaCl solutions (5, 10, 15, 20 and 25 g.L-1) were prepared using demineralized water, aquifer water and EPA medium. For the second objective, solutions of equal concentrations were prepared with salts obtained from the natural environment, previously sterilized, and dissolved in phreatic water. Survival was higher in the aquifer water, whose chemical composition was relatively more representative of the lakes inhabited by both species. It was almost complete at 20 and 15 g.L-1 for D. menucoensis and M. eugeniae, respectively. Lower survival of D. menucoensis was found in EPA medium, although it was developed for breeding other species of Daphnia. In the second series of bioassays, survival was almost complete until 20 g.L-1 (D. menucoensis) and 25 g.L-1 (M. eugeniae). The combination natural salts/aquifer water was adequate to assess the tolerance of both cladocerans. However, the former was more important, since the results were consistent with previous studies when these salts were dissolved in demineralized water. In addition, survival was much higher than when we used salts of analytical grade.},
 year = {2014}
}

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